PEO6 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

PEO6 cell line细胞株

Keywords: Human ovarian cancer; oestrogen receptor positive,
Cell Line Description: PEO6 is an adherent cell line derived from a malignant effusion from the peritoneal ascites of a patient with a poorly differentiated serous adenocarcinoma. The patient previously received cisplatin, 5-fluorouracil and chlorambucil treatment. PEO6 was collected at advanced, terminal stage disease. PEO6 is tumourigenic in immunologically-deprived CBA mice. PEO6 exhibits good growth in semi-solid medium (agar). PEO6 is from the same patient as the PEO1 and PEO4 cell lines (ECACC catalogue numbers 10032308 & 10032309).This cell line is one of nine from the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer, isolated from various malignant sites, and at various treatment stages) available at ECACC which provides a model system for research into the mechanism of oestrogen action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity of oestrogen protagonists.
Species: Human
Tissue of Origin: Ovary
CellType: Epithelial; islands of uniform polygonal cells.
Growth Mode: Adherent
DNA Profile: STR-PCR Data:
Amelogenin: X
CSF1PO: 10,12
D13S317: 10
D16S539: 9
D5S818: 11,12
D7S820: 10
THO1: 9.3
TPOX: 9,11
vWA: 15,16

Karyotype: For modal chromosome numbers see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72.
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time approximately 77 hours.
Culture Medium: RPMI 1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Foetal Bovine Serum (FBS).

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