Staphylococcus aureus ATCC 49525 (Xen36)荧光表达金黄色葡萄球菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Staphylococcus aureus ATCC 49525 (Xen36)荧光表达金黄色葡萄球菌株

Genetic Characteristics
Staphylococcus aureus Xen36 was derived from
the parental strain Staphylococcus aureus ATCC
49525 (Wright), a clinical isolate from bacteremia
patient. S. aureus Xen 36 possesses a stable copy
of the modified Photorhabdus luminescens
luxABCDE operon at a single integration site on a
native plasmid.
Growth Characteristics
S. aureus Xen36 grows well in various media
including Brain Heart Infusion (BHI), Trypticase
Soy Broth (TSB), and Luria Bertani (LB) at 37ºC
under ambient aeration. S. aureus Xen36 may also
be grown selectively on medium containing 200
μg/ml kanamycin.
Colonial Morphology
On TSA agar plate, S. aureus Xen36 appears as
small (~1.5mm), cream-colored, opaque, smooth,
circular colonies.
Virulence Factor
Capsule: Serotype 8.
DNAse: Positive.
NaCl: Tolerant via growth on Mannitol Salts
Agar.
Coagulase: Positive in 4hrs.
mecA: negative
Growth Curve
Log-phase growth can be achieved after 1.5 to 3
hours of subculture in TSB broth at 37ºC with
aeration at 200 rpm. An absorbance measurement
at 600 nm (against a TSB blank) of 0.5 is roughly
equivalent to 1.0x108 cfu/ml of S. aureus Xen36.

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