pTMP100 plasmid金黄色葡萄球菌自发荧光表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pTMP100 plasmid金黄色葡萄球菌自发荧光表达质粒载体

pTMP100pMK4 containing S. aureus PrpsJ:gfp:luxABCDEHill laboratory collectionpTRC99ArrnB T1, rrnB T227pDONRP4-P1REntry vector to clone attB4 and attB1 flanked PCR products, Kmr, Cmr, ccdB+InvitrogenpDONR221Entry vector to clone attB1 and attB2 flanked PCR products, Kmr, Cmr, ccdB+InvitrogenpDONR P2R-P3Entry vector to clone attB2 and attB3 flanked PCR products, Kmr, Cmr, ccdB+InvitrogenpDESTR4-R3Destination vector, contains attR4/R3 sites. Recombines with entry clones in a Multisite Gateway LR reaction, Apr, Cmr, ccdB+InvitrogenpHB201B. subtilis ori-pBR322, ori-1060, cat86:lacZα Cmr Emr15pMK4Gram-Positive Shuttle vector, ApR, Cmr28pSB2018pMK4 containing xylR PxylA:gfp8pSB2026pMK4 containing xylR PxylA:luxABCDEHill laboratory collectionpUNK1Gram-positive shuttle vector, Emr14pDEST-pMK4pMK4 containing attR4 and attR3 sites for recombination with entry clones in an LR reaction. Apr, Cmr, ccdB+This workpDEST-pUNK1pUNK1 containing attR4 and attR3 sites for recombination with entry clones in an LR reaction. Emr, Cmr, ccdB+This workpDEST-pHB201pHB201 containing attR4 and attR3 sites for recombination with entry clones in an LR reaction. Emr, Cmr, ccdB+This workpSB30003 fragment expression cassette in pMK4. xylR PxylA:gfp:rrnBT1T2 (0 attB)This workpSB30023 fragment expression cassette in pMK4. xylR PxylA:gfp:rrnBT1T2 (2 attB)This workpSB30043 fragment expression cassette in pMK4. xylR PxylA:gfp:rrnBT1T2 (4 attB)This workpSB30053 fragment expression cassette in MK4. PxylA:gfp:luxABCDE rrnBT1T2 (4 attB)This workpSB30073 fragment expression cassette in pUNK1. PxylA:gfp:luxABCDE:rrnBT1T2 (4 attB)This workpSB30103 fragment expression cassette in pMK4. xylR PxylA:luxABCDE:rrnBT1T2 (0 attB)This workpSB30123 fragment expression cassette in pMK4. xylR PxylA:luxABCDE:rrnBT1T2 (2 attB)This workpSB30143 fragment expression cassette in pMK4. xylR PxylA:luxABCDE:rrnBT1T2 (4 attB)This workpSB30243 fragment expression cassette in pHB201. PsspAgfp:luxABCDE:rrnBT1T2 (4 attB)

(3) (PDF) Construction and evaluation of multisite recombinatorial (Gateway) cloning vectors for Gram-positive bacteria. Available from: https://www.researchgate.net/publication/5961767_Construction_and_evaluation_of_multisite_recombinatorial_Gateway_cloning_vectors_for_Gram-positive_bacteria [accessed Sep 07 2021].

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