首页 » HeLaRC32 [HeRC32] cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

HeLaRC32 [HeRC32] cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥99865
  • 货  号:HeLaRC32 [HeRC32]
  • 产  地:北京
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HeLaRC32 [HeRC32] cell line细胞株

Product category
Human cells
Organism
Homo sapiens, human
Morphology
epithelial
Tissue
Uterus; Cervix
Disease
Adenocarcinoma
Applications
3D cell culture

Specific applications
These cells generate rAAV preparations with high titers of infectious particles which are essentially free of adenoviruses for biological, preclinical, clinical or pharmaceutical applications.
HelaRC32 is a stable packaging cell line expressing the rep and cap genes for recombinant adeno-associated virus type 2 (rAAV-2) assembly which constitutes an attractive alternative to transient transfection protocols.

Growth properties
Adherent
Derivation
This is a clone of HeLa cells that harbors one to two rep-cap gene copies per cell.
Age
31 years
Ethnicity
Black
Gender
Female
Genes expressed
Rep proteins: (Rep 78; Rep 68; Rep 52; and Rep 40); Cap proteins: (VP1; VP2; and VP3)
Comments
This is a clone of HeLa cells that harbors one to two rep-cap gene copies per cell. Upon vector transfection and adenovirus infection, efficient rAAV assembly correlated with a 100-fold amplification of the integrated rep-cap sequence with the inverted terminal repeats (ITRs) deleted.

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