pMY-IRES-GFP plasmid vector逆病毒荧光表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pMY-IRES-GFP plasmid vector逆病毒荧光表达质粒载体

Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. Most
retrovirus vectors including pBABE and pMXs are based on Moloney murine leukemia virus (MMLV).
MMLV-based vectors usually are silenced in immature cells including embryonic carcinoma (EC) cells
and embryonic stem (ES) cells, and possibly hematopoietic stem cells. Myeloproliferative sarcoma virus
(MPSV) and PCC4-cell-passaged myeloproliferative sarcoma virus (PCMV) are mutants of MMLV and
can stably express genes in immature cells including ES cells.
Cell Biolabs’ pMYs-IRES-GFP retroviral vector (also known as pMYs-IG) includes hybrid LTRs
containing elements from both MMLV and MPSV/PCMV, and it’s capable of expressing genes in
hematopoietic stem cells. The vector provides the viral package signal, transcription and processing
elements, and MCS for cloning of a target gene. The viral env gene, produced by the package cell line,
encodes the envelope protein, which determines the viral infectivity range. Transfection into a package
cell line produces high-titer, replication-incompetent viruses. In addition to transfer and expression of
exogenous genes in mammalian cells, recently, retroviruses have been used to express silencing RNAs
(siRNA) to decrease the expression of target genes both in vitro and in vivo.
The vector contains the ampicillin-resistance gene, LTRs, package signal and MCS for cloning of your
gene of interest

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