pMPE3 BioVector NTCC质粒载体菌株细胞基因保藏中心

Plasmid Name名称：pMPE3
Description描述：A plasmid pMPE3 was constructed by amplifying a 2.45 kb fragment of the Bacillus shuttle vector pMK4 (purchased from Bacillus Genetics Stock center) using the primers pUCCMNotIR (SEQ ID NO:88) and pUCCMNotIF (SEQ ID NO:89). The amplified fragment contained the multiple cloning site, lacZ-alpha, pMB1 origin of replication and chloramphenicol resistance gene from pMK4. The amplified fragment was digested with NotI and then circularized by ligation, generating pMPE3 which can replicate in E. coli but not in Lactobacillus plantarum.
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BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
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