pCass4-Rz plasmid vector植物双元表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥99865
- 货 号：pCass4-Rz
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pCass4-Rz plasmid vector植物双元表达质粒载体
The characteristic features of pCass4-RZ (Fig. 1A), a T-DNA plasmid amenable for agroinfiltration of plant cells and its derivatives harboring full-length cDNAs of BMV genomic RNAs are shown in Fig. 1B. Presence of a natural 5′ sequence is an essential prerequisite for efficient replication and wild-type (wt) biological activity of each of the three BMV genomic RNAs (Boyer and Haenni, 1994, Janda et al., 1987). Unlike natural 5′ end, the effect of 3′ non-viral nucleotide (nt) extensions on the biological activity of BMV RNAs varied significantly among the expression systems tested (Dreher et al., 1989, Dzianott and Bujarski, 1989, Ishikawa et al., 1997). Keeping these critical requirements in perspective, cloning of each of the three full-length cDNA copies of BMV genomic RNAs into pCass4-Rz binary vector (Fig. 1A) was designed to initiate the transcription by the cauliflower mosaic virus 35S promoter precisely at the authentic viral 5′ end (Fig. 1B). However, the possibility of having heterologous 5′ end sequence in transiently synthesized mRNAs due to the presence of double 35S promoter is not excluded. The 3′ end of each of the three de novo synthesized RNA transcripts will terminate with +22-nt extensions beyond the natural 3′ CCAOH due to the presence of a self-cleaving ribozyme
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
The characteristic features of pCass4-RZ (Fig. 1A), a T-DNA plasmid amenable for agroinfiltration of plant cells and its derivatives harboring full-length cDNAs of BMV genomic RNAs are shown in Fig. 1B. Presence of a natural 5′ sequence is an essential prerequisite for efficient replication and wild-type (wt) biological activity of each of the three BMV genomic RNAs (Boyer and Haenni, 1994, Janda et al., 1987). Unlike natural 5′ end, the effect of 3′ non-viral nucleotide (nt) extensions on the biological activity of BMV RNAs varied significantly among the expression systems tested (Dreher et al., 1989, Dzianott and Bujarski, 1989, Ishikawa et al., 1997). Keeping these critical requirements in perspective, cloning of each of the three full-length cDNA copies of BMV genomic RNAs into pCass4-Rz binary vector (Fig. 1A) was designed to initiate the transcription by the cauliflower mosaic virus 35S promoter precisely at the authentic viral 5′ end (Fig. 1B). However, the possibility of having heterologous 5′ end sequence in transiently synthesized mRNAs due to the presence of double 35S promoter is not excluded. The 3′ end of each of the three de novo synthesized RNA transcripts will terminate with +22-nt extensions beyond the natural 3′ CCAOH due to the presence of a self-cleaving ribozyme
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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