pREP3xV5 plasmid裂殖酵母表达载体Gateway质粒-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selection

NdeI site between V5 tag and attR1 element can be used to introduce additional tag(s) by conventional cloning. nmt1/nmt1*/nmt1** - thiamine repressible promoter wt/weak/very weak, correspondingly. 2xV5-attR1-CmR-ccdB-attR2 cassette was introduced into pREP-X vectors by XhoI sites

• Tag / Fusion Protein

  • double V5 epitope tag (N terminal on backbone)

  
  

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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