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FLO-1 cell line人食管腺癌细胞株
Keywords: Distal oesophageal adenocarcinomaCell Line Description: FLO-1 was established from a primary distal oesophageal adenocarcinoma in a 68 year-old Caucasian male in 1991. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancerSpecies: HumanTissue of Origin: OesophagusCellType: EpithelialGrowth Mode: AdherentDNA Profile: STR-PCR Data:Amelogenin: XCSF1PO: 11D13S317: 11D16S539: 12,13D5S818: 12,14D7S820: 8THO1: 6TPOX: 9,11vWA: 16Subculture Routine: Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 105 cells/cm2.Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Keywords: Distal oesophageal adenocarcinomaCell Line Description: FLO-1 was established from a primary distal oesophageal adenocarcinoma in a 68 year-old Caucasian male in 1991. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancerSpecies: HumanTissue of Origin: OesophagusCellType: EpithelialGrowth Mode: AdherentDNA Profile: STR-PCR Data:Amelogenin: XCSF1PO: 11D13S317: 11D16S539: 12,13D5S818: 12,14D7S820: 8THO1: 6TPOX: 9,11vWA: 16Subculture Routine: Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 105 cells/cm2.Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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