出品公司: | Invitrogen/ Ambion |
---|---|
载体名称: | pSilencer5.1-U6 Retro, pSilencer 5.1-U6 Retro, pSilencer5.1-U6-Retro |
质粒类型: | 哺乳动物细胞逆病毒基因沉默载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | U6 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 6486bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄 |
筛选标记: | 嘌呤霉素(Puromycin) |
备注: | 逆病毒基因沉默载体,能够将发卡的siRNA转染进入大多数哺乳细胞中。 |
产品目录号: | AM5784 |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 逆病毒 |
pSilencer5.1-U6 Retro逆病毒载体质粒图谱序列抗性价格报价Biovector NTCC Inc.
- 价 格:¥1920
- 货 号:BiovectorSilencer5.1-U6 Retro
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSilencer\5.1-U6 Retro 6486 bp DNA circular 6-OCT-2013 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|biovector.net| COMMENT VNTNAME|pSilencer 5.1-U6 Retro| BASE COUNT 1460 a 1840 c 1693 g 1493 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat ggtgcatgca aggagatggc 421 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat 481 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc 541 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggcgattagt 601 ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca 661 ccagctgaag cctatagagt acgagccata gataaaataa aagattttat ttagtctcca 721 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc 781 attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga 841 acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg 901 gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga 961 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac 1021 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa 1081 agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac 1141 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggactcg ctgatccttg 1201 ggagggtctc ctcagattga ttgactgccc acctcggggg tctttcattt ggaggttcca 1261 ccgagatttg gagacccctg cccagggacc accgaccccc ccgccgggag gtaagctggc 1321 cagcggtcgt ttcgtgtctg tctctgtctt tgtgcgtgtt tgtgccggca tctaatgttt 1381 gcgcctgcgt ctgtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac 1441 tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact ttgggggccg 1501 tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt 1561 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc 1621 ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg 1681 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc 1741 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt 1801 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt 1861 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt 1921 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc 1981 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc 2041 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc 2101 ctccctttat ccagccctca ctccttctct aggcgccgga attagatcga tctctcgagg 2161 tcgacggtat cgataagctt ttccaaaaaa gtactgctta cgatacggtc tcttgaaccg 2221 tatcgtaagc agtactggat cccgcgtcct ttccacaaga tatataaacc caagaaatcg 2281 aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact 2341 gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg 2401 tttacagtca aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat 2461 gaaggaatca tgggaaatag gccctcttcc tgcccgacct tggcgcgcgc tcggcgcgcg 2521 gtcacgctcc gtcacgtggt gcgttttgcc tgcgcgtctt tccactgggg aattctaccg 2581 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 2641 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 2701 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 2761 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 2821 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 2881 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 2941 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 3001 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 3061 ctcatctccg ggcctttcga cctgcagccc aagctagctt accatgaccg agtacaagcc 3121 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 3181 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 3241 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 3301 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 3361 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 3421 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 3481 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 3541 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 3601 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 3661 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc 3721 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ataaaataaa agattttatt 3781 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagagaa 3841 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact 3901 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa 3961 gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc 4021 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg 4081 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg taacagtttc 4141 ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat cctgactcaa 4201 ttagccactg ttttgaatcc acatactcca atactcctga aatagttcat tatggacagc 4261 gcagaaagag ctggggagaa ttgtgaaatt gttatccgct cacaattcca cacaacatac 4321 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 4381 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 4441 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 4501 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 4561 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 4621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 4681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 4741 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 4801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 4861 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 4921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 4981 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 5041 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 5101 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 5161 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 5221 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 5281 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 5341 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 5401 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 5461 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 5521 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 5581 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 5641 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 5701 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 5761 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 5821 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 5881 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 5941 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6001 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 6061 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 6121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 6181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 6241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 6301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 6361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 6421 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 6481 ttcgtc
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