| 出品公司: | Agilent |
|---|---|
| 载体名称: | pShuttle |
| GenBank序列号: | AF334399 |
| 质粒类型: | 腺病毒表达载体，哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6568 bp |
| 5' 测序引物及序列: | pShuttle-F: GAAGTGAAATCTGAATAATTTTGTG |
| 3' 测序引物及序列: | pShuttle-R: CAAAACTACATAAGACCCCCAC |
| 载体标签: | -- |
| 载体抗性: | 卡那 |
| 备注: | -- |
| 产品目录号: | 240006 |
| 稳定性: | / |
| 组成型: | -- |
| 病毒/非病毒: | 腺病毒 |
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载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
pShuttle contains only a multiple cloning site. This allows for the insertion of an entire expression cassette, including specialized promoters and termination signals (up to 7.5 kb). The regions indicated as arms are the stretches of sequence homology with pAdEasy-1 where the homologous recombination occurs. The R-ITR and L-ITR regions are short inverted terminal repeats (Left and Right) which have a role in replication of the viral DNA.
载体序列
LOCUS pShuttle 6568 bp DNA circular SYN 07-FEB-2001
DEFINITION AdEasy vector pShuttle, complete sequence.
ACCESSION AF334399
VERSION AF334399.1 GI:12698892
KEYWORDS .
SOURCE AdEasy vector pShuttle
ORGANISM AdEasy vector pShuttle
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6568)
AUTHORS Grafsky,A.J. III.
TITLE Direct Submission
JOURNAL Submitted (08-JAN-2001) Technical Services, Stratagene, 11011 N.
Torrey Pines Rd, La Jolla, CA 92037, USA
COMMENT This file is created by Vector NTI
http://www.biovector.net/
COMMENT VNTAUTHORNAME|biovector.net|
FEATURES Location/Qualifiers
source 1..6568
/organism="AdEasy vector pShuttle"
/mol_type="genomic DNA"
/db_xref="taxon:148367"
/lab_host="Escherichia coli"
/label="extrachromosomal
used for the cloning of cDNA to be recombined with the
pADEasy-1 vector; for infection of, and expression in,
various mammalian cell types"
LTR 4..106
/label="left inverted terminal repeat (LITR)"
misc_signal 197..345
/label="encapsidation"
primer_bind 313..337
/label="forward"
misc_feature 359..418
/label="MCS"
misc_feature 416..2414
/label="right arm homology region"
primer_bind 452..473
/label="reverse"
misc_feature 2682..3583
/label="left arm homology region"
LTR 3584..3686
/label="right inverted terminal repeat (RITR)"
rep_origin 3764..4542
/label="pBR322 ori"
CDS 5289..6083
/label="kanamycin resistance protein"
/codon_start=1
/transl_table=11
/product="neomycin phosphotransferase"
/protein_id="AAK01721.1"
/db_xref="GI:12698893"
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat actggtaccg cggccgcctc gagtctagag atatcgaatt
361 caagcttgtc gactcgaaga tctgggcgtg gttaagggtg ggaaagaata tataaggtgg
421 gggtcttatg tagttttgta tctgttttgc agcagccgcc gccgccatga gcaccaactc
481 gtttgatgga agcattgtga gctcatattt gacaacgcgc atgcccccat gggccggggt
541 gcgtcagaat gtgatgggct ccagcattga tggtcgcccc gtcctgcccg caaactctac
601 taccttgacc tacgagaccg tgtctggaac gccgttggag actgcagcct ccgccgccgc
661 ttcagccgct gcagccaccg cccgcgggat tgtgactgac tttgctttcc tgagcccgct
721 tgcaagcagt gcagcttccc gttcatccgc ccgcgatgac aagttgacgg ctcttttggc
781 acaattggat tctttgaccc gggaacttaa tgtcgtttct cagcagctgt tggatctgcg
841 ccagcaggtt tctgccctga aggcttcctc ccctcccaat gcggtttaaa acataaataa
901 aaaaccagac tctgtttgga tttggatcaa gcaagtgtct tgctgtcttt atttaggggt
961 tttgcgcgcg cggtaggccc gggaccagcg gtctcggtcg ttgagggtcc tgtgtatttt
1021 ttccaggacg tggtaaaggt gactctggat gttcagatac atgggcataa gcccgtctct
1081 ggggtggagg tagcaccact gcagagcttc atgctgcggg gtggtgttgt agatgatcca
1141 gtcgtagcag gagcgctggg cgtggtgcct aaaaatgtct ttcagtagca agctgattgc
1201 caggggcagg cccttggtgt aagtgtttac aaagcggtta agctgggatg ggtgcatacg
1261 tggggatatg agatgcatct tggactgtat ttttaggttg gctatgttcc cagccatatc
1321 cctccgggga ttcatgttgt gcagaaccac cagcacagtg tatccggtgc acttgggaaa
1381 tttgtcatgt agcttagaag gaaatgcgtg gaagaacttg gagacgccct tgtgacctcc
1441 aagattttcc atgcattcgt ccataatgat ggcaatgggc ccacgggcgg cggcctgggc
1501 gaagatattt ctgggatcac taacgtcata gttgtgttcc aggatgagat cgtcataggc
1561 catttttaca aagcgcgggc ggagggtgcc agactgcggt ataatggttc catccggccc
1621 aggggcgtag ttaccctcac agatttgcat ttcccacgct ttgagttcag atggggggat
1681 catgtctacc tgcggggcga tgaagaaaac ggtttccggg gtaggggaga tcagctggga
1741 agaaagcagg ttcctgagca gctgcgactt accgcagccg gtgggcccgt aaatcacacc
1801 tattaccggg tgcaactggt agttaagaga gctgcagctg ccgtcatccc tgagcagggg
1861 ggccacttcg ttaagcatgt ccctgactcg catgttttcc ctgaccaaat ccgccagaag
1921 gcgctcgccg cccagcgata gcagttcttg caaggaagca aagtttttca acggtttgag
1981 accgtccgcc gtaggcatgc ttttgagcgt ttgaccaagc agttccaggc ggtcccacag
2041 ctcggtcacc tgctctacgg catctcgatc cagcatatct cctcgtttcg cgggttgggg
2101 cggctttcgc tgtacggcag tagtcggtgc tcgtccagac gggccagggt catgtctttc
2161 cacgggcgca gggtcctcgt cagcgtagtc tgggtcacgg tgaaggggtg cgctccgggc
2221 tgcgcgctgg ccagggtgcg cttgaggctg gtcctgctgg tgctgaagcg ctgccggtct
2281 tcgccctgcg cgtcggccag gtagcatttg accatggtgt catagtccag cccctccgcg
2341 gcgtggccct tggcgcgcag cttgcccttg gaggaggcgc cgcacgaggg gcagtgcaga
2401 cttttgaggg cgtagagctt gggcgcgaga aataccgatt ccggggagta ggcatccgcg
2461 ccgcaggccc cgcagacggt ctcgcattcc acgagccagg tgagctctgg ccgttcgggg
2521 tcaaaaacca ggtttccccc atgctttttg atgcgtttct tacctctggt ttccatgagc
2581 cggtgtccac gctcggtgac gaaaaggctg tccgtgtccc cgtatacaga ctnnngttta
2641 aacgaattcn nntataaaat gcaaggtgct gctcaaaaaa tcaggcaaag cctcgcgcaa
2701 aaaagaaagc acatcgtagt catgctcatg cagataaagg caggtaagct ccggaaccac
2761 cacagaaaaa gacaccattt ttctctcaaa catgtctgcg ggtttctgca taaacacaaa
2821 ataaaataac aaaaaaacat ttaaacatta gaagcctgtc ttacaacagg aaaaacaacc
2881 cttataagca taagacggac tacggccatg ccggcgtgac cgtaaaaaaa ctggtcaccg
2941 tgattaaaaa gcaccaccga cagctcctcg gtcatgtccg gagtcataat gtaagactcg
3001 gtaaacacat caggttgatt catcggtcag tgctaaaaag cgaccgaaat agcccggggg
3061 aatacatacc cgcaggcgta gagacaacat tacagccccc ataggaggta taacaaaatt
3121 aataggagag aaaaacacat aaacacctga aaaaccctcc tgcctaggca aaatagcacc
3181 ctcccgctcc agaacaacat acagcgcttc acagcggcag cctaacagtc agccttacca
3241 gtaaaaaaga aaacctatta aaaaaacacc actcgacacg gcaccagctc aatcagtcac
3301 agtgtaaaaa agggccaagt gcagagcgag tatatatagg actaaaaaat gacgtaacgg
3361 ttaaagtcca caaaaaacac ccagaaaacc gcacgcgaac ctacgcccag aaacgaaagc
3421 caaaaaaccc acaacttcct caaatcgtca cttccgtttt cccacgttac gtaacttccc
3481 attttaagaa aactacaatt cccaacacat acaagttact ccgccctaaa acctacgtca
3541 cccgccccgt tcccacgccc cgcgccacgt cacaaactcc accccctcat tatcatattg
3601 gcttcaatcc aaaataaggt atattattga tgatnnntta attaaggatc cnnncggtgt
3661 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
3721 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
3781 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
3841 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
3901 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
3961 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
4021 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
4081 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
4141 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
4201 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
4261 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
4321 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
4381 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
4441 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
4501 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
4561 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
4621 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
4681 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
4741 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
4801 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
4861 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
4921 agttcgccag ttaatagttt gcgcaacgtt gttgnnnnnn aaaaaggatc ttcacctaga
4981 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
5041 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
5101 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
5161 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
5221 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat
5281 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
5341 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
5401 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
5461 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
5521 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
5581 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
5641 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
5701 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
5761 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
5821 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
5881 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
5941 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
6001 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
6061 gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag ctcatttttt
6121 aaccaatagg ccgaaatcgg caacatccct tataaatcaa aagaatagac cgcgataggg
6181 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
6241 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca
6301 agttttttgc ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga
6361 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
6421 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
6481 gcgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct
6541 taattaacat catcaataat atacctta
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