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MG1655大肠杆菌表达宿主菌，感受态细胞
Organism:
Escherichia coli (Migula) Castellani and Chalmers
Designations: MG1655
Isolation:
derived from existing strain (derived from parent strain W1485 by acridine
orange curing of the F plasmid)
Antigenic
Properties:
serotype OR:H48:K
Genotype:
F- lambda- ilvG- rfb-50 rph-1
Serotype: OR:H48:K
This strain was sequenced by the Blattner laboratory because it
approximates wild-type E. coli and "has been maintained as a laboratory
strain with minimal genetic manipulation, having only been cured of the
temperate bacteriophage lambda and F plasmid by means of ultraviolet
light and acridine orange, respectively." (Blattner, et al. 1997). The
mutations listed in the genotype are present in most K-12 strains and
were probably acquired early in the history of the laboratory strain. A
frameshift at the end of rph results in decreased pyrE expression and a
mild pyrimidine starvation, such that the strain grows 10 to 15% more
slowly in pyrimidine-free medium than in medium containing uracil
(Jensen 1993). The ilvG- mutation is a frameshift that knocks out
acetohydroxy acid synthase II (Lawther, et al. 1982). The rfb-50 mutation
is an IS5 insertion that results in the absence of O-antigen synthesis (Liu
and Reeves 1994).
MG1655 was derived and named by Mark Guyer from strain W1485,
which was derived in Joshua Lederberg's lab from a stab-culture
descendant of the original K-12 isolate. This original E. coli strain K-12
was obtained from a stool sample of a diphtheria patient in Palo Alto, CA
in 1922 (Bachmann, B., pp. 2460-2488 in Neidhardt et al.1996,
Escherichia coli and Salmonella: Cellular and Molecular Biology, ASM
Press).
Media and growth curves
MG1655 grows on LB and M9 minimal medium (+ Glucose + 1ug/ml
thiamine). In doing experiments with microarrays, we sought a medium
that was both defined and reproducible (unlike LB), yet supported fast
growth rates. We now use Neidhardt's MOPS-based rich defined medium
(MOPS-RDM). We also sought a commercially available rich defined
medium, and finding none tried to grow MG1655 on a medium sold for
mammalian cell culture. It grows quite well, but we decided to stick with
Neidhardt's medium because it shows a sharper transition to stationary
phase.Biovector NTCC,
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