H69AR cell line细胞株及完全培养基-BioVector NTCC保藏中心

[Cell Designation细胞株名称] H69AR

Product category

Human cells

Organism

Homo sapiens

Cell type

epithelial cell

Morphology

epithelial

Tissue

Lung

Disease

Carcinoma

Applications

3D cell culture

Cancer research

Characteristics

Growth properties

Adherent

Derivation

The multiple drug resistant cell line H69AR was established from NCI-H69 cells (NTCC HTB-119) that were grown in the presence of increasing concentrations of adriamycin (doxorubicin) over a total of 14 months.

Age

55 years

Ethnicity

White

Gender

Male

Tumorigenic

Yes;

Yes, in nude mice

Comments

The H69AR cell line is approximately 50-fold resistant to adriamycin as compared to the parental NCI-H69 cell line.

The monoclonal antibodies QCRL-1 (NTCC HB-11765) and QCRL-3 (NTCC HB-11766) will react with fixed H69AR cells.

The cell line is cross-resistant to anthracycline analogues including daunomycin, epirubicin, menogaril, and mitoxantrone as well as to acivicin, etoposide, gramicidin D, colchicine, and the Vinca alkaloids, vincristine and vinblastine.

The cells display little or no cross-resistance to bleomycin, 5-fluorouracil, and carboplatin. They have a slight collateral sensitivity to 1-dehydrotestosterone and lidocaine.

The antibodies reacts with MRP (multidrug resistance protein), a 190,000 integral membrane phosphoglycoprotein that is overexpressed in some drug-selected resistant cell lines

Handling information

Unpacking and storage instructions

1.Check all containers for leakage or breakage.

2.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.

Complete medium

The base medium for this cell line is NTCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Temperature

37°C

Handling procedure

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

1.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).

2.Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.

3.It is recommended that the cryoprotective agent be removed immediately.  Centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes.  Discard the supernatant and resuspend the cell pellet in an appropriate amount of fresh growth medium.

4.Transfer the vial contents to an appropriate size vessel.  It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).

5.Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.

Quality control specifications

Mycoplasma contamination

Not detected

Population doubling time

Approximately 24 hrs

[Comments相关资料] "Group: Haploid karyotype cell line. Knockout cell: Method=CRISPR/Cas9; HGNC; 4408; GNG5. Sequence variation: Gene fusion; HGNC; 76; ABL1 + HGNC; 1014; BCR; Name(s)=BCR-ABL1, BCR-ABL (from parent cell line). Derived from sampling site: Bone marrow."

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
网址：www.biovector.net