pAAV8-CAG-GFP plasmid腺相关病毒载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pAAV8-CAG-GFP plasmid腺相关病毒载体质粒

Vector backboneAAV with CAG promoter

Backbone manufacturerScott Sternson
Backbone size w/o insert (bp)4722
Vector typeMammalian Expression, AAV ; Adeno-associated virus
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin
Growth Temperature30°C
Growth Strain(s)NEB Stable
Growth instructionsUse recombinase-free E. coli (Stbl3, XL-10, Sure2 and DH5a et.al), grow at 30C
Copy numberLow Copy
GENE/INSERT
Gene/Insert nameGFP
Alt nameGreen fluorescent protein
SpeciesA. victoria
Insert Size (bp)720
MutationN/A
PromoterCAG
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteBamHI (not destroyed)
3′ cloning siteEcoRI (not destroyed)
5′ sequencing primeraaagagacagcaaccagg
3′ sequencing primercggcttctggcgtgtgac

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net