NTC868系列蔗糖筛选质粒载体RNA-OUT plasmid BioVector NTCC质粒载体菌种细胞基因保藏中心

NTC868系列蔗糖筛选质粒载体RNA-OUT plasmid

Sucrose selection with RNA-OUT selection marker encoding Nature Technology Corporation (NTC) NTC868 series targeting DNA vaccine plasmids. Left: Sucrose selection. NTC868 series plasmid borne 70 bp RNA-OUT antisense RNA hybridizes to a RNA-IN target sequence in the leader of a constitutively expressed mRNA. This inhibits levansucrase (sacB) translation, allowing survival in sucrose media. Right: NTC868 series targeting vectors. The pUC replication origin is the purple arrow while the RNA-OUT selection marker is the brown arrow. The CMV promoter, transgene and rabbit globin polyA are depicted with orange arrow, blue arrow and green box, respectively. The transgene is shown, with the insertion point for the N-terminal secretion (NTC8682) proteasome (NTC8684) or endosome (NTC8681) targeting peptides indicated. Republished with permission of Humana Press from Luke et al. 2014

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