tsDC cell line永生化小鼠树突状细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

tsDC cell line永生化小鼠树突状细胞株

Cell Line Name: tsDC
Keywords: Immortalised mouse dendritic cell line

Cell Line Description: This is a conditionally immortalised dendritic cell line which was established from bone marrow of CBA (H-2k) mice transgenic for a thermo labile mutant of the SV40 large T antigen under the control of the Class I Kb promoter. At 33-37°C it divides in the absence of GM-CSF. It shares a number of cell surface markers with bone marrow macrophages, but unlike macrophages is constitutively MHC Class II+. Transfer to 39°C, arrests growth and results in up-regulation of surface markers such as B7.1, CD40 and intercellular adhesion molecule-1. Mycoplasma eradicated prior to deposit at ECACC.
Species: Mouse
Tissue of Origin: Bone marrow
Growth Mode: Semi-adherent
Karyotype: Not specified
GMO Status: Genetically Modified Organism Class 1 (GMO1)

Subculture Routine: Passage cells approximately every 1- 2 weeks. Culture at 33°C; 5-10% CO2. Cells have heterogenous morphology, but the line has been cloned. Most cells adhere but some also grow in suspension. Split sub-confluent cultures (70-80%) . Collect any suspension cells, then remove the attached cells via a PBS wash followed by short incubation in 20mM EDTA. Once the cells have detached add culture medium in excess and centrifuge at 150g for 5 minutes.Seed new flasks at 1-3 x10,000 cells/cm².  
If resuscitating from a frozen ampoule seed at approximately 4 x10,000 cells/cm².


Culture Medium: Iscove's MEM + 5% Foetal Bovine Serum (FBS) + 2 mM Glutamine + 0.05 mM 2Mercaptoethanol (2ME).

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