UMUC1 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

UMUC1 cell line细胞株

Human bladder transitional cell carcinoma
Cell Line Description: Human transitional cell carcinoma (grade II) derived from a bladder cancer metastasis of a black male. Metastatic involvement of lymphatics. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Species: Human
Tissue of Origin: Bladder
CellType: Epithelial
Growth Mode: Adherent
DNA Profile: STR-PCR Data:
Amelogenin: X
CSF1PO: 12
D13S317: 12,14
D16S539: 9,11
D5S818: 13
D7S820: 8,11
THO1: 8
TPOX: 8
vWA: 14,15
Subculture Routine: Split subconfluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Doubling time approximately 26 hours.
Culture Medium: MEM + 1% NEAA + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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