NCI H82 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

NCI H82 cell line细胞株

Organism Homo sapiens, humanTissue lung; derived from metastatic site: pleural effusionProduct Format frozenMorphology epithelialCulture Properties aggregates in suspension; the cells grow in very large aggregates, and the aggregates are the only viable cell populationBiosafety Level 1cDisease carcinoma; small cell lung cancerAge 40 yearsGender maleEthnicity CaucasianKaryotype This is a near triploid human cell line. The modal chromosome number is 58, occurring at 44% with polyploidy at 3%. Marker chromosomes der(1)t(1;709p13;p11), t(13q;?HSR;15q) and der(190t(19;?)(q13.4;?) were common to most cells., There were two distinct subpopulations readily distinguished by karyotype. Besides uniform changes in the numbers of copies of some normal chromosomes, one population had der(3)t(3;20)(p11;p11?), t(3q19p), i(7q) and a minute chromosome of unknown origin., The other had t(1q17p), del(1)(q21), der(3)t(3;7)(p12;q11) plus two other markers. Each cell had two copies of a normal X chromosome. The Y chromosome was not detected in Q banded preparations.Derivation The NCI-H82 cell line was derived by A.F. Gazdar and associates in 1978 from the pleural fluid of a patient with small cell cancer of the lung.Derived from metastatic site, pleural effusionClinical Data 40 yearsCaucasianmaleThe NCI-H82 cell line was derived by A.F. Gazdar and associates in 1978 from the pleural fluid of a patient with small cell cancer of the lung.Receptor Expression Insulin-like growth factor II (IGF II); atrial natriuretic peptide (ANP)Oncogene myc +; myb -; raf +; ras +; fms +; fes +Tumorigenic YesEffects Yes, in nude miceComments The morphology of the original tumor was not characteristic of SCLC.The line is a biochemical and morphological variant of SCLC that expresses neuron specific enolase and the brain isoenzyme of creatine kinase.It does not have detectable levels of L-DOPA decarboxylase or bombesin.The cells produce an abnormally sized p53 mRNA (3.7 kb).C-myc DNA sequences are amplified about 25 fold, and there is a 24 fold increase in c-myc RNA relative to normal cells.The cells are reported to express functional ANP receptors, but treatment with ANP does not alter their growth pattern.The cells stain positively for neurofilaments and vimentin.There is expression of v-fes, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs.Complete Growth Medium The base medium for this cell line is NTCC-formulated RPMI-1640 Medium, NTCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (NTCC 30-2020) to a final concentration of 10%.Subculturing This line grows as aggregates of cells in suspension. Culture can be maintained by addition of medium or by replacement of medium. Alternatively, the cells may be collected by centrifugation and dispersed into fresh medium.Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommendedMedium Renewal: 2 to 3 times per weekCryopreservation Culture medium, 95%; DMSO, 5%Culture Conditions Temperature: 37°CSTR Profile Amelogenin: XCSF1PO: 11D13S317: 8D16S539: 12D5S818: 12D7S820: 10,13TH01: 9,9.3TPOX: 11vWA: 14Isoenzymes AK-1, 1ES-D, 1G6PD, BGLO-I, 1Me-2, 1PGM1, 1-2PGM3, 1-2Year of Origin 1978References Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201

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