the murine macrophages cell line J774A.1细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

the murine macrophages cell line J774A.1细胞株

Keywords: Mouse BALB/c monocyte macrophage Cell Line Description: Derived from a tumour in a female BALB/c mouse. Cell line produces IL-1 and lysozyme. Species: Mouse Tissue of Origin: Blood Growth Mode: Semi-adherent Karyotype: Not specified Subculture Routine: Cells are subcultured by harvesting attached and suspension cells. Remove majority of medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Seed into new flask at 1:3 to 1:4 split ratio i.e. seeding at 1-3 x 10,000 cells/cm2 Incubate at 37°C; 5% CO2. Please Note :- On receipt growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet. This should then be re-suspended in fresh media and a cell count performed. The cells should then be reseeded at 2-4 x 10E4/cm and should begin to show signs of re-attachment within 48 hours. Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Depositor: Obtained from ATCC Originator: No Country: USA References: Cancer Res 1977;37:546; J Immunol 1977;119:950; J Immunol 1975;114:898

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