d283 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

d283 cell line细胞株

Organism Homo sapiens, human Tissue brain/cerebellum; derived from metastatic site: peritoneum Cell Type Medulloblastoma Product Format frozen Morphology epithelial Culture Properties suspension, multicell aggregates and some adherent cells Biosafety Level 1 Disease medulloblastoma Age 6 years juvenile Gender male Ethnicity Caucasian Karyotype The karyotype is 45, XY, -7, -8, -17, -20, der(20)t(1;20)(q12;q13), 8q+, 17p+ (range = 41 to 46). This is a hypodiploid cell line with a frequency of higher ploidies of 5.4%. Three marker chromosomes are present in all cells. They are: der(20)t(1;20)(q12;q13), 8q+ and 17p+. N7, N17 and N20 have single copies. The single X is structurally normal, and the Y chromosome is present as confirmed by fluorescence microscopy. Derivation The D283 Med cell line was established in 1985 by Friedman et al. from malignant ascites cells and a peritoneal metastasis from a boy with medulloblastoma. _x001f_ Derived from metastatic site, peritoneum Clinical Data 6 years juvenile Caucasian The D283 Med cell line was established in 1985 by Friedman et al. from malignant ascites cells and a peritoneal metastasis from a boy with medulloblastoma. male Genes Expressed The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity. Cellular Products neurofilament proteins (NFP) Tumorigenic Yes Effects Yes, in nude mice (The cells form serially transplantable intercranial and subcutaneous tumors.) Yes, in soft agar Comments The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. Complete Growth Medium The base medium for this cell line is NTCC-formulated Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Cultures can be maintained by addition or replacement of medium. Adherent cells can be dislodged by scraping, and cultures can be established by resuspending a cell pellet at 5 X 105viable cells/mL. Maintain cultures between 4 X 104 and 8 X 105 cells/mL. Medium Renewal: 2 to 3 times per week Cryopreservation Freeze medium: culture medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase Culture Conditions Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% STR Profile Amelogenin: X,Y CSF1PO: 9,12 D13S317: 8,10 D16S539: 11 D5S818: 11 D7S820: 10 TH01: 7 TPOX: 8,11 vWA: 16,18 Isoenzymes AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1 PGM3, 1 Population Doubling Time 52 hrs Year of Origin 1985 References Friedman HS, et al. Phenotypic and genotypic analysis of a human meduloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am. J. Pathol. 130: 472-484, 1988. PubMed: 3279793

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