pLOW plasmid金黄色葡萄球菌表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pLOW plasmid金黄色葡萄球菌表达质粒载体

Plasmid pLOW, designed for regulated expression of S. aureus proteins. The MCS is shown in full, with the reading frame indicated by the triplet arrangement of the nucleotide sequence. Only unique restriction sites are shown. Genes: ApR, E. coli blaM gene for b-lactamase; ColEI-HC, high-copy-number E. coli plasmid origin of replication; EmR, S. aureus gene originating from plasmid pE194 and encoding Em-resistance gene ermC; pSK41 ori, lowcopy-number S. aureus origin of replication; LacI, lac repressor protein constitutively expressed from the penicillinase promoter Ppcn. (b) Plasmid pLOW-GFP, designed for producing protein– GFP fusions in S. aureus. GFP, gfpmut-1 gene encoding GFP.  

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