首页 » CHOZN GS-/- ZFN BioVector NTCC质粒载体菌种细胞基因保藏中心

CHOZN GS-/- ZFN BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥986835
  • 货  号:CHOZN GS-/- ZFN
  • 产  地:北京
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CHOZN GS-/- ZFN

General descriptionCHOZN cell lines were created utilizing Sigma′s proprietary CompoZr Zinc Finger Nuclease (ZFN) technology to create genetic modifications in parental CHO-K1 cells. Cells are banked in chemically defined, animal component free EX-CELL CD CHO Fusion mediaZFNs are a class of engineered DNA-binding proteins and causes a double-strand break. This double-strand break is necessary for site-specific mutagenesis mediated DNA repair. It facilitates targeted genome editing by binding to a user-specified locus.[1] Glutamine synthetase (GS) is one of the most commonly used selectable markers in the biopharmaceutical industry. Glutamine is an essential amino acid for cellular growth.[2] The GS enzyme is responsible for the conversion of glutamate into glutamine.[3] Without this enzymatic activity, cells can no longer synthesize glutamine endogenously and if glutamine is not supplemented in the culture media, the cells will die.Cell Line OriginCHOZN cell lines are all subclones of the parental CHO-K1 cell line originated by Puck in 1957.

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