出品公司: | Addgene |
---|---|
载体名称: | pShuttle-CMV-EGFP-C |
质粒类型: | 腺病毒载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 8156 bp |
5' 测序引物及序列: | GGCACCAAAATCAACGGGACTTTCC |
3' 测序引物及序列: | -- |
载体标签: | EGFP |
载体抗性: | Kanamycin |
筛选标记: | -- |
备注: | 克隆菌株:DH5alpha |
产品目录号: | 13887 |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | 腺病毒 |
pShuttle-CMV-EGFP-C腺病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc.
- 价 格:¥1920
- 货 号:BiovectorShuttle-CMV-EGFP-C
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pShuttle-CMV-EGFP-C 8156 bp DNA SYN DEFINITION pShuttle-CMV-EGFP-C ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..8156 /organism="pShuttle-CMV-EGFP-C" /mol_type="other DNA" misc_feature complement(27..105) /label="RITR" misc_feature 27..105 /label="LITR" misc_feature 185..333 /label="Encap" promoter 349..901 /label="CMV_immearly_promoter" misc_feature 404..691 /label="CAG_enhancer" misc_feature 858..878 /label="CMV_fwd_primer" promoter 859..928 /label="CMV_promoter" CDS 972..1763 /label="ORF frame 3" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*" gene 975..1688 /label="EGFP" /gene="EGFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*" misc_feature complement(1017..1038) /label="EGFP_N_primer" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*" misc_feature 1155..1184 /label="Y66 (EGFP)" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*" misc_feature 1625..1646 /label="EGFP_C_primer" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*" terminator 1805..2033 /label="SV40_PA_terminator" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*" misc_feature 2003..2022 /label="EBV_rev_primer" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*" CDS complement(2609..3967) /label="ORF frame 2" /translation="MLPGRRAGRRPAALQHQQDQPQAHPGQRAARSAPLHRDPDYADE DPAPVERHDPGPSGRAPTTAVQRKPPQPAKRGDMLDRDAVEQVTELWDRLELLGQTLK SMPTADGLKPLKNFASLQELLSLGGERLLADLVRENMRVRDMLNEVAPLLRDDGSCSS LNYQLHPVIGVIYGPTGCGKSQLLRNLLSSQLISPTPETVFFIAPQVDMIPPSELKAW EMQICEGNYAPGPDGTIIPQSGTLRPRFVKMAYDDLILEHNYDVSDPRNIFAQAAARG PIAIIMDECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDMAGN IANLKIQSKMHLISPRMHPSQLNRFVNTYTKGLPLAISLLLKDIFRHHAQRSCYDWII YNTTPQHEALQWCYLHPRDGLMPMYLNIQSHLYHVLEKIHRTLNDRDRWSRAYRARKT PK*" CDS complement(3715..4236) /label="ORF frame 3" /translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP NPRNEEICWIEMP*" misc_feature complement(5194..5289) /label="LITR" /translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP NPRNEEICWIEMP*" misc_feature 5194..5289 /label="RITR" /translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP NPRNEEICWIEMP*" rep_origin complement(5534..6153) /label="pBR322_origin" /translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP NPRNEEICWIEMP*" gene complement(6308..6607) /label="Ampicillin (299 - 0)" /gene="Ampicillin (299 - 0)" /translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP NPRNEEICWIEMP*" promoter 6810..6859 /label="NEOKAN_promoter" /translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP NPRNEEICWIEMP*" CDS 6948..7742 /label="ORF frame 3" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 6951..7739 /label="NeoR/KanR" /gene="NeoR/KanR" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" ORIGIN 1 NNTCCCTTCC AGCTCTCTGC CCCTTTTGGA TTGAAGCCAA TATGATAATG AGGGGGTGGA 61 GTTTGTGACG TGGCGCGGGG CGTGGGAACG GGGCGGGTGA CGTAGTAGTG TGGCGGAAGT 121 GTGATGTTGC AAGTGTGGCG GAACACATGT AAGCGACGGA TGTGGCAAAA GTGACGTTTT 181 TGGTGTGCGC CGGTGTACAC AGGAAGTGAC AATTTTCGCG CGGTTTTAGG CGGATGTTGT 241 AGTAAATTTG GGCGTAACCG AGTAAGATTT GGCCATTTTC GCGGGAAAAC TGAATAAGAG 301 GAAGTGAAAT CTGAATAATT TTGTGTTACT CATAGCGCGT AANNNNTAAT AGTAATCAAT 361 TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA 421 TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT 481 TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA 541 AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT 601 CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC 661 TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA 721 GTACATCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT 781 TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA 841 CAACTCCGCC CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 901 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGAGATCg atcTGGTACC GTCGACGTCG 961 CGGCCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG 1021 TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG 1081 ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC 1141 CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG 1201 ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC 1261 GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG 1321 GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA 1381 TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA 1441 AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG 1501 TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 1561 CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG 1621 ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC 1681 TGTACAAGTC CGGACTCAGA TCTGTAGGTG CATTAATGGA AGTGATGCAG AAGAGGAGCA 1741 AAGCCATTCA TTCTTCAGAC TGAAGCTTCT AGATAAGATA TCCGATCCAC CGGATCTAGA 1801 TAACTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC 1861 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT 1921 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA 1981 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGGA 2041 Tctgggcgtg gtTAAGGGTG GGAAAGAATA TATAAGGTGG GGGTCTTATG TAGTTTTGTA 2101 TCTGTTTTGC AGCAGCCGCC GCCGCCATGA GCACCAACTC GTTTGATGGA AGCATTGTGA 2161 GCTCATATTT GACAACGCGC ATGCCCCCAT GGGCCGGGGT GCGTCAGAAT GTGATGGGCT 2221 CCAGCATTGA TGGTCGCCCC GTCCTGCCCG CAAACTCTAC TACCTTGACC TACGAGACCG 2281 TGTCTGGAAC GCCGTTGGAG ACTGCAGCCT CCGCCGCCGC TTCAGCCGCT GCAGCCACCG 2341 CCCGCGGGAT TGTGACTGAC TTTGCTTTCC TGAGCCCGCT TGCAAGCAGT GCAGCTTCCC 2401 GTTCATCCGC CCGCGATGAC AAGTTGACGG CTCTTTTGGC ACAATTGGAT TCTTTGACCC 2461 GGGAACTTAA TGTCGTTTCT CAGCAGCTGT TGGATCTGCG CCAGCAGGTT TCTGCCCTGA 2521 AGGCTTCCTC CCCTCCCAAT GCGGTTTAAA ACATAAATAA AAAACCAGAC TCTGTTTGGA 2581 TTTGGATCAA GCAAGTGTCT TGCTGTCTTT ATTTAGGGGT TTTGCGCGCG CGGTAGGCCC 2641 GGGACCAGCG GTCTCGGTCG TTGAGGGTCC TGTGTATTTT TTCCAGGACG TGGTAAAGGT 2701 GACTCTGGAT GTTCAGATAC ATGGGCATAA GCCCGTCTCT GGGGTGGAGG TAGCACCACT 2761 GCAGAGCTTC ATGCTGCGGG GTGGTGTTGT AGATGATCCA GTCGTAGCAG GAGCGCTGGG 2821 CGTGGTGCCT AAAAATGTCT TTCAGTAGCA AGCTGATTGC CAGGGGCAGG CCCTTGGTGT 2881 AAGTGTTTAC AAAGCGGTTA AGCTGGGATG GGTGCATACG TGGGGATATG AGATGCATCT 2941 TGGACTGTAT TTTTAGGTTG GCTATGTTCC CAGCCATATC CCTCCGGGGA TTCATGTTGT 3001 GCAGAACCAC CAGCACAGTG TATCCGGTGC ACTTGGGAAA TTTGTCATGT AGCTTAGAAG 3061 GAAATGCGTG GAAGAACTTG GAGACGCCCT TGTGACCTCC AAGATTTTCC ATGCATTCGT 3121 CCATAATGAT GGCAATGGGC CCACGGGCGG CGGCCTGGGC GAAGATATTT CTGGGATCAC 3181 TAACGTCATA GTTGTGTTCC AGGATGAGAT CGTCATAGGC CATTTTTACA AAGCGCGGGC 3241 GGAGGGTGCC AGACTGCGGT ATAATGGTTC CATCCGGCCC AGGGGCGTAG TTACCCTCAC 3301 AGATTTGCAT TTCCCACGCT TTGAGTTCAG ATGGGGGGAT CATGTCTACC TGCGGGGCGA 3361 TGAAGAAAAC GGTTTCCGGG GTAGGGGAGA TCAGCTGGGA AGAAAGCAGG TTCCTGAGCA 3421 GCTGCGACTT ACCGCAGCCG GTGGGCCCGT AAATCACACC TATTACCGGG TGCAACTGGT 3481 AGTTAAGAGA GCTGCAGCTG CCGTCATCCC TGAGCAGGGG GGCCACTTCG TTAAGCATGT 3541 CCCTGACTCG CATGTTTTCC CTGACCAAAT CCGCCAGAAG GCGCTCGCCG CCCAGCGATA 3601 GCAGTTCTTG CAAGGAAGCA AAGTTTTTCA ACGGTTTGAG ACCGTCCGCC GTAGGCATGC 3661 TTTTGAGCGT TTGACCAAGC AGTTCCAGGC GGTCCCACAG CTCGGTCACC TGCTCTACGG 3721 CATCTCGATC CAGCATATCT CCTCGTTTCG CGGGTTGGGG CGGCTTTCGC TGTACGGCAG 3781 TAGTCGGTGC TCGTCCAGAC GGGCCAGGGT CATGTCTTTC CACGGGCGCA GGGTCCTCGT 3841 CAGCGTAGTC TGGGTCACGG TGAAGGGGTG CGCTCCGGGC TGCGCGCTGG CCAGGGTGCG 3901 CTTGAGGCTG GTCCTGCTGG TGCTGAAGCG CTGCCGGTCT TCGCCCTGCG CGTCGGCCAG 3961 GTAGCATTTG ACCATGGTGT CATAGTCCAG CCCCTCCGCG GCGTGGCCCT TGGCGCGCAG 4021 CTTGCCCTTG GAGGAGGCGC CGCACGAGGG GCAGTGCAGA CTTTTGAGGG CGTAGAGCTT 4081 GGGCGCGAGA AATACCGATT CCGGGGAGTA GGCATCCGCG CCGCAGGCCC CGCAGACGGT 4141 CTCGCATTCC ACGAGCCAGG TGAGCTCTGG CCGTTCGGGG TCAAAAACCA GGTTTCCCCC 4201 ATGCTTTTTG ATGCGTTTCT TACCTCTGGT TTCCATGAGC CGGTGTCCAC GCTCGGTGAC 4261 GAAAAGGCTG TCCGTGTCCC CGTATACAGA CTNNNGTTTA AACGAATTCN NNATATAAAA 4321 TGCAAGGTGC TGCTCAAAAA ATCAGGCAAA GCCTCGCGCA AAAAAGAAAG CACATCGTAG 4381 TCATGCTCAT GCAGATAAAG GCAGGTAAGC TCCGGAACCA CCACAGAAAA AGACACCATT 4441 TTTCTCTCAA ACATGTCTGC GGGTTTCTGC ATAAACACAA AATAAAATAA CAAAAAAACA 4501 TTTAAACATT AGAAGCCTGT CTTACAACAG GAAAAACAAC CCTTATAAGC ATAAGACGGA 4561 CTACGGCCAT GCCGGCGTGA CCGTAAAAAA ACTGGTCACC GTGATTAAAA AGCACCACCG 4621 ACAGCTCCTC GGTCATGTCC GGAGTCATAA TGTAAGACTC GGTAAACACA TCAGGTTGAT 4681 TCATCGGTCA GTGCTAAAAA GCGACCGAAA TAGCCCGGGG GAATACATAC CCGCAGGCGT 4741 AGAGACAACA TTACAGCCCC CATAGGAGGT ATAACAAAAT TAATAGGAGA GAAAAACACA 4801 TAAACACCTG AAAAACCCTC CTGCCTAGGC AAAATAGCAC CCTCCCGCTC CAGAACAACA 4861 TACAGCGCTT CACAGCGGCA GCCTAACAGT CAGCCTTACC AGTAAAAAAG AAAACCTATT 4921 AAAAAAACAC CACTCGACAC GGCACCAGCT CAATCAGTCA CAGTGTAAAA AAGGGCCAAG 4981 TGCAGAGCGA GTATATATAG GACTAAAAAA TGACGTAACG GTTAAAGTCC ACAAAAAACA 5041 CCCAGAAAAC CGCACGCGAA CCTACGCCCA GAAACGAAAG CCAAAAAACC CACAACTTCC 5101 TCAAATCGTC ACTTCCGTTT TCCCACGTTA CGTAACTTCC CATTTTAAGA AAACTACAAT 5161 TCCCAACACA TACAAGTTAC TCCGCCCTAA AACCTACGTC ACCCGCCCCG TTCCCACGCC 5221 CCGCGCCACG TCACAAACTC CACCCCCTCA TTATCATATT GGCTTCAATC CAAAATAAGG 5281 TATATTATTG ATGATNNNTT AATTAAGGAT CCNNNCGGTG TGAAATACCG CACAGATGCG 5341 TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 5401 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 5461 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 5521 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 5581 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 5641 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 5701 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 5761 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 5821 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 5881 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 5941 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 6001 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 6061 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 6121 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 6181 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 6241 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 6301 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 6361 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 6421 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 6481 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 6541 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 6601 TGCGCAACGT TGTTGNNNNA AAAAGGATCT TCACCTAGAT CCTTTTCACG TAGAAAGCCA 6661 GTCCGCAGAA ACGGTGCTGA CCCCGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG 6721 AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG CGATAGCTAG 6781 ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC AGCTGGGGCG CCCTCTGGTA 6841 AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTC GCCGCCAAGG ATCTGATGGC 6901 GCAGGGGATC AAGCTCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG 6961 ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG 7021 CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC 7081 CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA GACGAGGCAG 7141 CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA 7201 CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT 7261 CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA 7321 CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC 7381 GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC 7441 TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC GAGGATCTCG 7501 TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG 7561 GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA 7621 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG 7681 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT 7741 GAATTTTGTT AAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC 7801 AACATCCCTT ATAAATCAAA AGAATAGACC GCGATAGGGT TGAGTGTTGT TCCAGTTTGG 7861 AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT 7921 CAGGGCGATG GCCCACTACG TGAACCATCA CCCAAATCAA GTTTTTTGCG GTCGAGGTGC 7981 CGTAAAGCTC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG 8041 CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG 8101 GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CGCGCTTAAT GCGCCG //
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载体应用举例
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