pUFR034 plasmid vector黄单胞杆菌穿梭质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pUFR034 plasmid vector黄单胞杆菌穿梭质粒载体


Designations  pUFR034
Depositors  DW Gabriel
Biosafety Level  1
Vector Information  
Size (kb): 8.6999998092651370
Vector: pUFR034 (cosmid)
Promoters: Promoter lac
Construction: pUC18, pSa747, pHC79, pUCD550, pUC4Kan, pLAFR3
Marker(s):neoR
Construct size (kb): 8.699999809265137
Features: insert detection: lacZ'
marker(s): neoR
promoter: lac
replicon: oriW
Applications  
shuttle vector
vector for constructing genomic libraries
vector permitting visual detection of recombinants
Comments  
Restriction digests of the clone give the following sizes (kb): EcoRI--8.7; PstI--6.4, 1.35, 1.2; SmaI--7.0, 1.8.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Uses interruption of lacZalpha.
Shuttle vector permitting visual detection of recombinants.
The partition locus from Agrobacterium plasmid pTAR stabilizes the plasmid in X. citri and X. campestris pvs. campestris, vesicatoria, malvacearum, and translucens grown in broth culture.
The order of the major features in this plasmid is: lacZalpha with MCS (pUC18) - oriW (pSa747, IncW) - cos (pHC79) - parA+ (pUCD550) - aphA (pUC4Kan) - mob(P) (pLAFR3, Mob+).
Media  ATCC® Medium 1799: LB medium (ATCC medium 1065) plus 10 mcg/ml neomycin
Growth Conditions  
Temperature: 30.0°C
References  

DeFeyter R, et al. Small, stable shuttle vectors for use in Xanthomonas. Gene 88: 65-72, 1990. PubMed: 2341039

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