KBM-7 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

KBM-7 cell line细胞株

KBM-7 cells were derived from a 39-year-old man with chronic myeloid leukemia in blast crisis. The original cell line contained both near haploid and hyperdiploid clones. Subsequent subcloning yielded a pure near-haploid cell line.Genome analysis has revealed that besides the disomic chromosome 8 also a 30 megabase fragment of chromosome 15 is present in two copies. Like other CML cells lines (e.g., K562) KBM-7 cells are positive for the Philadelphia chromosome harboring the BCR-ABL oncogenic fusion. KBM-7 cells have been reprogrammed to yield the HAP1 cell line which is also haploid for chromosome 8.
Product Type: Knockout Stable Cell Line Cat#: NTCC-C7637 Product Name: Human KBM-7 cell line Size: 1 vial Description: Human KBM-7 cell line is Quality-controlled near-haploid KBM-7 wild-type cells Host Species: Human Biosafety Level: 1 Growth Properties: Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed 2x106 cells per ml in pre-warmed media in a 10cm dish. Recommended Medium: Growth Medium: IMDM, 10% FCS; Freeze Medium: IMDM, 20% FCS, 10% DMSO; Revival: Cells are suspension cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, seeding 2x106 cells per ml.

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