1618-K cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

1618-K cell line细胞株

Cell line: 1618-K NTCC no.: ACC 752 Species: human (Homo sapiens) Cell type: testicular germ cell tumor Origin: established in 1981 from the cytoreductive thoracotomy specimen of a man with primary retroperitoneal embryonal carcinoma after 9 cycles of cisplatin-based chemotherapy Reference(s): 18015 Biosafety level: 1 Permissions and restrictions: A NTCC Cell Culture Data: Morphology: epithelioid cells growing adherently in monolayers; image; image Medium: 80% RPMI 1640 + 20% h.i. FBS Subculture: seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture every 4-6 days using trypsin/EDTA Incubation: at 37 °C with 5% CO2 Doubling time: ca. 40 hours Harvest: cell harvest of ca. 5 x 106 cells/80 cm2 Storage: frozen with 70% medium, 20% FBS, 10% DMSO NTCC Scientific Data: Mycoplasma: negative in PCR assay Immunology: To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@NTCC.de. Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

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