pWY121 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：pWY121 plasmid vector质粒载体
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pWY121 plasmid vector质粒载体
Recombination vector pWY121FEATURES Location/Qualifiers source 1..9447 /organism="Recombination vector pWY121" /mol_type="other DNA" /db_xref="taxon:1147124" regulatory 44..312 /regulatory_class="terminator" /note="tL3" gene complement(980..1765) /gene="bet" CDS complement(980..1765) /gene="bet" /codon_start=1 /transl_table=11 /product="Bet recombinase" /protein_id="AFA52011.1" /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA" gene complement(1762..2475) /gene="cI857" CDS complement(1762..2475) /gene="cI857" /codon_start=1 /transl_table=11 /product="repressor protein" /protein_id="AFA52012.1" /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG QSGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY EYPVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE SCSVVGKVIASQWPEETFG" regulatory 2492..2520 /regulatory_class="promoter" /note="PRM" regulatory 2533..2561 /regulatory_class="promoter" /note="PR" gene 2590..3621 /gene="cre" CDS 2590..3621 /gene="cre" /note="P1 phage cyclization recombinase" /codon_start=1 /transl_table=11 /product="Cre recombinase" /protein_id="AFA52013.1" /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN LAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAK DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA MVRLLEDGD" gene 6978..7712 /gene="ermC" CDS 6978..7712 /gene="ermC" /note="ErmC" /codon_start=1 /transl_table=11 /product="erythromycin resistance determinant" /protein_id="AFA52014.1" /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF TLELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNI PYNISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPR EYFHPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH AGIDDLNNISFEQFLSLFNSYKLFNK"
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Recombination vector pWY121FEATURES Location/Qualifiers source 1..9447 /organism="Recombination vector pWY121" /mol_type="other DNA" /db_xref="taxon:1147124" regulatory 44..312 /regulatory_class="terminator" /note="tL3" gene complement(980..1765) /gene="bet" CDS complement(980..1765) /gene="bet" /codon_start=1 /transl_table=11 /product="Bet recombinase" /protein_id="AFA52011.1" /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA" gene complement(1762..2475) /gene="cI857" CDS complement(1762..2475) /gene="cI857" /codon_start=1 /transl_table=11 /product="repressor protein" /protein_id="AFA52012.1" /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG QSGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY EYPVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPS FPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE SCSVVGKVIASQWPEETFG" regulatory 2492..2520 /regulatory_class="promoter" /note="PRM" regulatory 2533..2561 /regulatory_class="promoter" /note="PR" gene 2590..3621 /gene="cre" CDS 2590..3621 /gene="cre" /note="P1 phage cyclization recombinase" /codon_start=1 /transl_table=11 /product="Cre recombinase" /protein_id="AFA52013.1" /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN LAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAK DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA MVRLLEDGD" gene 6978..7712 /gene="ermC" CDS 6978..7712 /gene="ermC" /note="ErmC" /codon_start=1 /transl_table=11 /product="erythromycin resistance determinant" /protein_id="AFA52014.1" /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF TLELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNI PYNISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPR EYFHPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH AGIDDLNNISFEQFLSLFNSYKLFNK"
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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