pNIRUBI43 pSUP202 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pNIRUBI43 pSUP202 plasmid vector质粒载体

R. eapsulatus BIOS wild-type Smr (32) BSRUB13 .1.glnK This work PHU332 glnB::Kmr P. Hübner, Basel RCAY22 amtY::Gmr (62) RCAY63 amtB::Kmr (62) SG21 glnK::Gmr This work TR3 glnB-Y51F 013528::Gmr This work Plasmids pAP2 pJB3TC20 derivative carrying glnK-Y51 F and 300 bp of This work upstream sequence pAY98 pMECA derivative carrying glnKamtB; Apr A. Yakunin pBSRUB13 pNIRUB145 derivative carrying laeZ' expressed from a This work Km cassette; Gmr pBSRUB19 pPHU231 derivative carrying amtB and most of glnK This work pBSRUB21-1 pBSRUB 19 derivative carrying amtBC expressed from a This work Km cassette pET-22b(+) T7 expression vector; Apr Novagen pJB3TC20 Broad-host range vector; mob Apr Tc' (5) pK18 Cloning vector; Kmr (46) pML5 Broad-host-range laeZ-fusion vector; mob Tc' (35) pNIRUB60-I pUC18 derivative carrying aphll (Kmr ) of transposon Tn5 This work pNIRUB142 pML5 derivative carrying laeZ' expressed from a Km cassette pNIRUBI43 pSUP202 derivative carrying most of amtB This work pNIRUB144 pNIRUB 143 derivative carrying glnK::Gmr amtB This work pNIRUB145 pNIRUBI44 derivative with a 228 bp deletion in glnK This work pPHU231 Broad-host range vector; mob Ter (47) pSG5IIa pUC 18 derivative carrying glnK::Gmr ; Apr This work pSGI8 pK18 derivative carrying most of amtB This work pSGNJl pSVB 1 0 derivative carrying glnK-amtB; Apr This work pSUP202 Mobilizablc cloning vcctor; mob Tc' Apr Cmr (50) pSV5 pET-22b(+) derivative carrying glnBhis6 This work pSV6 pET-22b(+) dcrivative carrying glnKhis6 This work pSVBIO Cloning vector; Apr (2) pTR9 pK18 derivative carrying 5' part of glnB and 013528 This work pTRI0 pUC 18 derivative carrying 3' part of glnB This work pTR11 pTR9 derivative carrying Gmr from pWKR440 inserted This work into 013528 pTRI2 pTR 1 0 derivative; mob Tc' This work pTR14-1 pTR 12 derivative carrying glnB-Y51 F and 013528::Gmr This work pUC18 Cloning vector, Apr (55) pWKR56-1 Mobilizable cloning vector; mob Tc' (32) pWKR440 pACYC derivative carrying GmrTc' (Il)

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