2E8 cell line小鼠骨髓瘤细胞生产免疫球蛋白表面IgM BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：2E8 cell line小鼠骨髓瘤细胞生产免疫球蛋白表面IgM
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2E8 cell line小鼠骨髓瘤细胞生产免疫球蛋白表面IgM
Organism Mus musculus, mouse Tissue bone marrow Cell Type B lymphocyte Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Age immature Strain BALB/c.xid Applications The 2E8 line is dependent on IL-7, and can be used for detecting and quantifying IL-7.Antigen Expression Antigen expression: H-2 d; Ia d; BP-1 Receptor Expression interleukin 7 (IL-7) Genes Expressed immunoglobulin (surface IgM) Cellular Products immunoglobulin (surface IgM) Comments The 2E8 line is dependent on IL-7, and can be used for detecting and quantifying IL-7.Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol, 1 ng/ml mouse interleukin-7 and 20% fetal bovine serum Subculturing Every 2 to 3 days, the cells must be diluted without centrifugation at a concentration of 5 X 105 viable cells/mL by adding fresh medium to the culture. Mouse IL-7 can be obtained from several commercial sources including Upstate Biotechnology, Inc., Lake Placid, NY (1-800-548-7853). The cells do not grow as well when human IL-7 is substituted for mouse IL-7. Medium Renewal: Every 2 to 4 days Cryopreservation Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase Culture Conditions Temperature: 37°C Isotype IgM , IgMReferences Pietrangeli CE, et al. Stromal cell lines which support lymphocyte growth: characterization, sensitivity to radiation and responsiveness to growth factors. Eur. J. Immunol. 18: 863-872, 1988. PubMed: 3260182 Ishihara K, et al. Stromal-cell and cytokine-dependent lymphocyte clones which span the pre-B- to B-cell transition. Dev. Immunol. 1: 149-161, 1991. PubMed: 1821694
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Organism Mus musculus, mouse Tissue bone marrow Cell Type B lymphocyte Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Age immature Strain BALB/c.xid Applications The 2E8 line is dependent on IL-7, and can be used for detecting and quantifying IL-7.Antigen Expression Antigen expression: H-2 d; Ia d; BP-1 Receptor Expression interleukin 7 (IL-7) Genes Expressed immunoglobulin (surface IgM) Cellular Products immunoglobulin (surface IgM) Comments The 2E8 line is dependent on IL-7, and can be used for detecting and quantifying IL-7.Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol, 1 ng/ml mouse interleukin-7 and 20% fetal bovine serum Subculturing Every 2 to 3 days, the cells must be diluted without centrifugation at a concentration of 5 X 105 viable cells/mL by adding fresh medium to the culture. Mouse IL-7 can be obtained from several commercial sources including Upstate Biotechnology, Inc., Lake Placid, NY (1-800-548-7853). The cells do not grow as well when human IL-7 is substituted for mouse IL-7. Medium Renewal: Every 2 to 4 days Cryopreservation Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase Culture Conditions Temperature: 37°C Isotype IgM , IgMReferences Pietrangeli CE, et al. Stromal cell lines which support lymphocyte growth: characterization, sensitivity to radiation and responsiveness to growth factors. Eur. J. Immunol. 18: 863-872, 1988. PubMed: 3260182 Ishihara K, et al. Stromal-cell and cytokine-dependent lymphocyte clones which span the pre-B- to B-cell transition. Dev. Immunol. 1: 149-161, 1991. PubMed: 1821694
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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