BJAB cell line人B细胞淋巴瘤细胞株ACC 757 BioVector NTCC质粒载体菌种细胞基因保藏中心

BJAB cell line人B细胞淋巴瘤细胞株ACC 757

Cell line:
BJAB
DSMZ no.:
ACC 757
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established in 1973 from the inguinal tumor of an EBV-negative 5-year-old African girl with Burkitt lymphoma at the preterminal stage; although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
round to polymorphic cells growing in suspension singly cells and in clumps;
Images:


Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5-0.7 x 106 cells/ml; optimal split ratio 1:2 -1:5 every 2-3 days; culture flask should be kept horizontal
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image
Fingerprint:
genetic typing showed a pattern of short tandem repeats (STR) matching the reference profile of the international STR database
Species:
human
Cytogenetics:
human hypodiploid karyotype; 44(40-46)<2n>XX, -10, -21, der(6)t(6;10)(p22;p12), der(7)t(1;7)(p36;p22), t(11;17)(q24;q24), der(11)t(11;17)(q24;q24)t(11;6)(p15;q11)t(6;5)(q22;?), ider(13)(q10)t(3;13)(q26;q14), der(13)t(10;13)(q11;p11)dup(13)(q11q32)t(13;21)(q32;q22), der(21)t(13;21); does not correspond with previous BJA-B karyotypes published; no IG/MYC translocation detected
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net