pAdTrack-CMV腺病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc.

LOCUS       pAdTrack-CMV	9220 bp 	DNA     circular	SYN
DEFINITION  pAdTrack-CMV
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biovector.net/
COMMENT     VNTAUTHORNAME|biovector.net|
FEATURES             Location/Qualifiers
     source          1..9044
                     /organism="pAdTrack-CMV"
                     /mol_type="other DNA"
     misc_feature    196..344
                     /label="Encap"
     misc_feature    complement(373..392)
                     /label="EBV_rev_primer"
     CDS             574..1353
                     /label="ORF frame 1"
     CDS             complement(592..1338)
                     /label="ORF frame 3"
     gene            complement(622..1335)
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(664..685)
                     /label="EGFP_C_primer"
     misc_feature    complement(1126..1155)
                     /label="Y66 (EGFP)"
     misc_feature    1272..1293
                     /label="EGFP_N_primer"
     misc_feature    complement(1412..1432)
                     /label="CMV_fwd_primer"
     misc_feature    complement(1963..1982)
                     /label="EBV_rev_primer"
     promoter        complement(2332..2403)
                     /label="CMV_promoter"
     misc_feature    complement(2382..2402)
                     /label="CMV_fwd_primer"
     misc_feature    complement(2568..2843)
                     /label="CAG_enhancer"
     CDS             complement(3462..4820)
                     /label="ORF frame 1"
     CDS             complement(4568..5089)
                     /label="ORF frame 2"
     misc_feature    complement(6046..6141)
                     /label="LITR"
     misc_feature    6046..6141
                     /label="RITR"
     rep_origin      complement(6386..7005)
                     /label="pBR322_origin"
     gene            complement(7160..7459)
                     /label="Ampicillin (299 - 0)"
                     /gene="Ampicillin (299 - 0)"
     promoter        7664..7713
                     /label="NEOKAN_promoter"
     CDS             7802..8596
                     /label="ORF frame 2"
     gene            7805..8593
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
ORIGIN
    1 NNNTTAATTA ANNNTCCCTT CCAGCTCTCT GCCCCTTTTG GATTGAAGCC AATATGATAA
   61 TGAGGGGGTG GAGTTTGTGA CGTGGCGCGG GCGTGGGAAC GGGGCGGGTG ACGTAGTAGT
  121 GTGGCGGAAG TGTGATGTTG CAAGTGTGGC GGAACACATG TAAGCGACGG ATGTGGCAAA
  181 AGTGACGTTT TTGGTGTGCG CCGGTGTACA CAGGAAGTGA CAATTTTCGC GCGGTTTTAG
  241 GCGGATGTTG TAGTAAATTT GGGCGTAACC GAGTAAGATT TGGCCATTTT CGCGGGAAAA
  301 CTGAATAAGA GGAAGTGAAA TCTGAATAAT TTTGTGTTAC TCATAGCGCG TAANNNCGCG
  361 TTAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT
  421 ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA
  481 GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT
  541 TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCA GTTATCTAGA
  601 TCCGGTGGAT CTGAGTCCGG ACTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC
  661 GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC
  721 GGACTGGGTG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT
  781 GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT GGCGGATCTT
  841 GAAGTTCACC TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT TGTGGCTGTT
  901 GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT
  961 CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA
 1021 GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA
 1081 CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG CTGAAGCACT GCACGCCGTA
 1141 GGTCAGGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATGAA
 1201 CTTCAGGGTC AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT
 1261 GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGAACAGCTC
 1321 CTCGCCCTTG CTCACCATGG TGGCGACCGG TAGCGCTAGC GGATCTGACG GTTCACTAAA
 1381 CCAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG
 1441 GCGGAGTTGT TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCAAAA CAAACTCCCA
 1501 TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA TCCACGCCCA
 1561 TTGATGTACT GCCAAAACCG CATCACCATG GTAATAGCGA TGACTAATAC GTAGATGTAC
 1621 TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC GGGCCATTTA
 1681 CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG TACTGCCAAG
 1741 TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC TATTGGCGTT
 1801 ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG CGGTCAGCCA
 1861 GGCGGGCCAT TTACCGTAAG TTATGTAACG CGGAACTCCA TATATGGGCT ATGAACTAAT
 1921 GACCCCGTAA TTGATTACTA TTANNNCGCG TTAAGATACA TTGATGAGTT TGGACAAACC
 1981 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
 2041 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG
 2101 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT
 2161 GGTATGGCTG ATTATGATCA GTTATCTAGA TCCGGTGGAT CTGAGTCCGG ACTTGTACAG
 2221 CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCANNGATC GGATATCTTA
 2281 TCTAGAAGCT TAGGCTCGAG CGGCCGCGTC GACGGTACCA GATCTCTAGC GGATCTGACG
 2341 GTTCACTAAA CCAGCTCTGC TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG
 2401 CGTCAATGGG GCGGAGTTGT TACGACATTT TGGAAAGTCC CGTTGATTTT GGTGCCAAAA
 2461 CAAACTCCCA TTGACGTCAA TGGGGTGGAG ACTTGGAAAT CCCCGTGAGT CAAACCGCTA
 2521 TCCACGCCCA TTGATGTACT GCCAAAACCG CATCACCATG GTAATAGCGA TGACTAATAC
 2581 GTAGATGTAC TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAT AATGCCAGGC
 2641 GGGCCATTTA CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG
 2701 TACTGCCAAG TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC
 2761 TATTGGCGTT ACTATGGGAA CATACGTCAT TATTGACGTC AATGGGCGGG GGTCGTTGGG
 2821 CGGTCAGCCA GGCGGGCCAT TTACCAACGC GGAACTCCAT ATATGGGCTA TGAACTAATG
 2881 ACCCCGTAAT TGATTACTAT TANNNTAAGG GTGGGAAAGA ATATATAAGG TGGGGGTCTT
 2941 ATGTAGTTTT GTATCTGTTT TGCAGCAGCC GCCGCCGCCA TGAGCACCAA CTCGTTTGAT
 3001 GGAAGCATTG TGAGCTCATA TTTGACAACG CGCATGCCCC CATGGGCCGG GGTGCGTCAG
 3061 AATGTGATGG GCTCCAGCAT TGATGGTCGC CCCGTCCTGC CCGCAAACTC TACTACCTTG
 3121 ACCTACGAGA CCGTGTCTGG AACGCCGTTG GAGACTGCAG CCTCCGCCGC CGCTTCAGCC
 3181 GCTGCAGCCA CCGCCCGCGG GATTGTGACT GACTTTGCTT TCCTGAGCCC GCTTGCAAGC
 3241 AGTGCAGCTT CCCGTTCATC CGCCCGCGAT GACAAGTTGA CGGCTCTTTT GGCACAATTG
 3301 GATTCTTTGA CCCGGGAACT TAATGTCGTT TCTCAGCAGC TGTTGGATCT GCGCCAGCAG
 3361 GTTTCTGCCC TGAAGGCTTC CTCCCCTCCC AATGCGGTTT AAAACATAAA TAAAAAACCA
 3421 GACTCTGTTT GGATTTGGAT CAAGCAAGTG TCTTGCTGTC TTTATTTAGG GGTTTTGCGC
 3481 GCGCGGTAGG CCCGGGACCA GCGGTCTCGG TCGTTGAGGG TCCTGTGTAT TTTTTCCAGG
 3541 ACGTGGTAAA GGTGACTCTG GATGTTCAGA TACATGGGCA TAAGCCCGTC TCTGGGGTGG
 3601 AGGTAGCACC ACTGCAGAGC TTCATGCTGC GGGGTGGTGT TGTAGATGAT CCAGTCGTAG
 3661 CAGGAGCGCT GGGCGTGGTG CCTAAAAATG TCTTTCAGTA GCAAGCTGAT TGCCAGGGGC
 3721 AGGCCCTTGG TGTAAGTGTT TACAAAGCGG TTAAGCTGGG ATGGGTGCAT ACGTGGGGAT
 3781 ATGAGATGCA TCTTGGACTG TATTTTTAGG TTGGCTATGT TCCCAGCCAT ATCCCTCCGG
 3841 GGATTCATGT TGTGCAGAAC CACCAGCACA GTGTATCCGG TGCACTTGGG AAATTTGTCA
 3901 TGTAGCTTAG AAGGAAATGC GTGGAAGAAC TTGGAGACGC CCTTGTGACC TCCAAGATTT
 3961 TCCATGCATT CGTCCATAAT GATGGCAATG GGCCCACGGG CGGCGGCCTG GGCGAAGATA
 4021 TTTCTGGGAT CACTAACGTC ATAGTTGTGT TCCAGGATGA GATCGTCATA GGCCATTTTT
 4081 ACAAAGCGCG GGCGGAGGGT GCCAGACTGC GGTATAATGG TTCCATCCGG CCCAGGGGCG
 4141 TAGTTACCCT CACAGATTTG CATTTCCCAC GCTTTGAGTT CAGATGGGGG GATCATGTCT
 4201 ACCTGCGGGG CGATGAAGAA AACGGTTTCC GGGGTAGGGG AGATCAGCTG GGAAGAAAGC
 4261 AGGTTCCTGA GCAGCTGCGA CTTACCGCAG CCGGTGGGCC CGTAAATCAC ACCTATTACC
 4321 GGGTGCAACT GGTAGTTAAG AGAGCTGCAG CTGCCGTCAT CCCTGAGCAG GGGGGCCACT
 4381 TCGTTAAGCA TGTCCCTGAC TCGCATGTTT TCCCTGACCA AATCCGCCAG AAGGCGCTCG
 4441 CCGCCCAGCG ATAGCAGTTC TTGCAAGGAA GCAAAGTTTT TCAACGGTTT GAGACCGTCC
 4501 GCCGTAGGCA TGCTTTTGAG CGTTTGACCA AGCAGTTCCA GGCGGTCCCA CAGCTCGGTC
 4561 ACCTGCTCTA CGGCATCTCG ATCCAGCATA TCTCCTCGTT TCGCGGGTTG GGGCGGCTTT
 4621 CGCTGTACGG CAGTAGTCGG TGCTCGTCCA GACGGGCCAG GGTCATGTCT TTCCACGGGC
 4681 GCAGGGTCCT CGTCAGCGTA GTCTGGGTCA CGGTGAAGGG GTGCGCTCCG GGCTGCGCGC
 4741 TGGCCAGGGT GCGCTTGAGG CTGGTCCTGC TGGTGCTGAA GCGCTGCCGG TCTTCGCCCT
 4801 GCGCGTCGGC CAGGTAGCAT TTGACCATGG TGTCATAGTC CAGCCCCTCC GCGGCGTGGC
 4861 CCTTGGCGCG CAGCTTGCCC TTGGAGGAGG CGCCGCACGA GGGGCAGTGC AGACTTTTGA
 4921 GGGCGTAGAG CTTGGGCGCG AGAAATACCG ATTCCGGGGA GTAGGCATCC GCGCCGCAGG
 4981 CCCCGCAGAC GGTCTCGCAT TCCACGAGCC AGGTGAGCTC TGGCCGTTCG GGGTCAAAAA
 5041 CCAGGTTTCC CCCATGCTTT TTGATGCGTT TCTTACCTCT GGTTTCCATG AGCCGGTGTC
 5101 CACGCTCGGT GACGAAAAGG CTGTCCGTGT CCCCGTATAC AGACTNNNGT TTAAACGAAT
 5161 TCNNNTATAA AATGCAAGGT GCTGCTCAAA AAATCAGGCA AAGCCTCGCG CAAAAAAGAA
 5221 AGCACATCGT AGTCATGCTC ATGCAGATAA AGGCAGGTAA GCTCCGGAAC CACCACAGAA
 5281 AAAGACACCA TTTTTCTCTC AAACATGTCT GCGGGTTTCT GCATAAACAC AAAATAAAAT
 5341 AACAAAAAAA CATTTAAACA TTAGAAGCCT GTCTTACAAC AGGAAAAACA ACCCTTATAA
 5401 GCATAAGACG GACTACGGCC ATGCCGGCGT GACCGTAAAA AAACTGGTCA CCGTGATTAA
 5461 AAAGCACCAC CGACAGCTCC TCGGTCATGT CCGGAGTCAT AATGTAAGAC TCGGTAAACA
 5521 CATCAGGTTG ATTCATCGGT CAGTGCTAAA AAGCGACCGA AATAGCCCGG GGGAATACAT
 5581 ACCCGCAGGC GTAGAGACAA CATTACAGCC CCCATAGGAG GTATAACAAA ATTAATAGGA
 5641 GAGAAAAACA CATAAACACC TGAAAAACCC TCCTGCCTAG GCAAAATAGC ACCCTCCCGC
 5701 TCCAGAACAA CATACAGCGC TTCACAGCGG CAGCCTAACA GTCAGCCTTA CCAGTAAAAA
 5761 AGAAAACCTA TTAAAAAAAC ACCACTCGAC ACGGCACCAG CTCAATCAGT CACAGTGTAA
 5821 AAAAGGGCCA AGTGCAGAGC GAGTATATAT AGGACTAAAA AATGACGTAA CGGTTAAAGT
 5881 CCACAAAAAA CACCCAGAAA ACCGCACGCG AACCTACGCC CAGAAACGAA AGCCAAAAAA
 5941 CCCACAACTT CCTCAAATCG TCACTTCCGT TTTCCCACGT TACGTAACTT CCCATTTTAA
 6001 GAAAACTACA ATTCCCAACA CATACAAGTT ACTCCGCCCT AAAACCTACG TCACCCGCCC
 6061 CGTTCCCACG CCCCGCGCCA CGTCACAAAC TCCACCCCCT CATTATCATA TTGGCTTCAA
 6121 TCCAAAATAA GGTATATTAT TGATGATNNN TTAATTAAGG ATCCNNNCGG TGTGAAATAC
 6181 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCTC TTCCGCTTCC TCGCTCACTG
 6241 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA
 6301 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC
 6361 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC
 6421 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT
 6481 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC
 6541 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT
 6601 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG
 6661 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC
 6721 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA
 6781 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA
 6841 GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA
 6901 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC
 6961 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG
 7021 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA
 7081 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG
 7141 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT
 7201 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG
 7261 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC
 7321 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA
 7381 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC
 7441 CAGTTAATAG TTTGCGCAAC GTTGTTGNNN NNNAAAAAGG ATCTTCACCT AGATCCTTTT
 7501 CACGTAGAAA GCCAGTCCGC AGAAACGGTG CTGACCCCGG ATGAATGTCA GCTACTGGGC
 7561 TATCTGGACA AGGGAAAACG CAAGCGCAAA GAGAAAGCAG GTAGCTTGCA GTGGGCTTAC
 7621 ATGGCGATAG CTAGACTGGG CGGTTTTATG GACAGCAAGC GAACCGGAAT TGCCAGCTGG
 7681 GGCGCCCTCT GGTAAGGTTG GGAAGCCCTG CAAAGTAAAC TGGATGGCTT TCTCGCCGCC
 7741 AAGGATCTGA TGGCGCAGGG GATCAAGCTC TGATCAAGAG ACAGGATGAG GATCGTTTCG
 7801 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
 7861 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
 7921 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
 7981 GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
 8041 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
 8101 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
 8161 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
 8221 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
 8281 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA
 8341 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
 8401 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA
 8461 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT
 8521 CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT
 8581 TGACGAGTTC TTCTGAATTT TGTTAAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT
 8641 AGGCCGAAAT CGGCAACATC CCTTATAAAT CAAAAGAATA GACCGCGATA GGGTTGAGTG
 8701 TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC
 8761 GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCAAA TCAAGTTTTT
 8821 TGCGGTCGAG GTGCCGTAAA GCTCTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG
 8881 CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG
 8941 GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCGCGCT
 9001 TAATGCGCCG NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 9061 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 9121 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 9181 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 
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