L-363 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

L-363 cell line细胞株


Cell line:
L-363
DSMZ no.:
ACC 49
Species:
human (Homo sapiens)
Cell type:
plasma cell leukemia
Origin:
established from the peripheral blood of a 36-year-old woman with plasma cell leukemia (IgG) in 1977; cells were described in the literature to be EBNA-negative and to express mRNA for proto-oncogene BCL2; plasma cell leukemia is related to multiple myeloma. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
Reference(s):
14461, 18187
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
single, large, round or oval cells in suspension; image; image; image; image
Medium:
85% RPMI 1640 + 15% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.2-0.5 x 106 cells/ml; split 1:2 to 1:3 every 3-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
maximum density at 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed originallyas human with IEF of AST, LDH, PEP B and later by STR fingerprinting
Cytogenetics:
human hyperdiploid karyotype with 11% polyploidy - 49(45-50)<2n>X, -X, -X, -6, +7, +8, +11, +11, +14, +19, -22, t(5;8)(q12/13;q24.32), der(8)(t(5;8)(q12/13;q24.32), der(8)t(7;8)(q11.2;p22), del(11)(q13)x2, add(14)(q32), del(17)(p12), add(19)(q13) - sideline with add(13)(q34) - 11q13 and 14q32 breakpoints recurrent in PCL - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -


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