JJN-3 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

JJN-3 cell line细胞株


Cell line:
JJN-3
DSMZ no.:
ACC 541
Species:
human (Homo sapiens)
Cell type:
plasma cell leukemia
Origin:
established from the bone marrow of a 57-year-old woman with plasma cell leukemia (IgA1kappa) at diagnosis in 1987; JJN-3 is a subclone of the parental cell line JJN-1; plasma cell leukemia is related to multiple myeloma
Reference(s):
14486, 14635
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
single round to oval cells showing a morphological range from mononuclear to occasionally multinucleated cells growing in suspension; image; image
Medium:
40% Dulbecco's MEM + 40% Iscove's MDM + 20% h.i. FBS
Subculture:
seed out at ca. 0.2-0.4 x 106 cells/ml; split saturated culture 1:3 to 1:6 every 2-3 days
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, cykappa +, sm/cylambda -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human flat-moded hypotriploid karyotype with 9% polyploidy - 58-67<3n>XX, +1, -2, +5, +8, +8, -9, -11, -12, -13, -15, -17, +20, add(1)(p22), der(1)t(1;?3)(q41;p26)x2, add(3)(p26), add(5)(p15)x1-2, i(5p), del(6)(q13),del(7)(q32), der(7)t(7;11)(q36;q13), add(8)(p1?)x2, t(12;19)(p13;q13), der(14)add(14)(p11)t(14;16)(q32;q23), der(14)t(14;16)(q32;q23), der(16)t(14;16)(q32;q23)x2, del(22)(q12) - carries two copies of t(14;16) associated with c-MAF activation - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

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