KYSE-270 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

KYSE-270 cell line细胞株


Cell line:
KYSE-270
DSMZ no.:
ACC 380
Species:
human (Homo sapiens)
Cell type:
esophageal squamous cell carcinoma
Origin:
derived from well differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 79-year-old Japanese man prior to treatment (depth of invasion was not beyond the muscularis propria); described in the literature to carry p53 mutation
Reference(s):
14954, 14955, 15006, 14646
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
epitheloid cells growing in monolayers; image; image; image
Medium:
49% RPMI 1640 + 49% Ham's F12 + 2% h.i. FBS
Subculture:
seed out at ca. 0.5-2 x 106 cells/80 cm2; split confluent culture 1:5 every second day using trypsin/EDTA (incubation for 10-15 min)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24 hours
Harvest:
cell harvest of ca. 10 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed originally as human with IEF of LDH, MDH, NP and later by STR typing
Cytogenetics:
human hypotriploid karyotype with 16% polyploidy - 66(64-67)<3n>XX/XXX, +1, -2, +3, -5, -6, +7, +8, -9, -10, -15, -15, -17, -18, -19, -20, -22, +5mar (1bisat), ?del(X)(q25), add(1)(p1?), add(1)(p2?), i(1p), der(1)t(1;?)(?;2)(q13;?)(?;q11), add(2)(q37), del(2)(q23), add(3)(p2?6)x2, i(5p), add(5)(p15), del(7)(p11), add(8)(q24), add(16)(q24), add(18)(q23), add(19)(p13), add(20)(q12), add(21)(p11)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV -


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