WEA枯草芽孢杆菌食品级表达菌株Bacillus subtilis strain BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥69835
- 货 号:WEA枯草芽孢杆菌食品级表达菌株Bacillus subtilis strain
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
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WEA枯草芽孢杆菌食品级表达菌株Bacillus subtilis strain
For expressing thegene of interest under food grade conditions,two B. subtilisstrains are available; TEA and WEA. Both strainscontain the toxin expression cassette ydcEunder control of the xylose-inducible promoter Pxyl, whereas the former ydcDEoperon (containing the toxin and antitoxin gene)is deleted.The strain B. subtilisTEA is based on B. subtilis168 Marburg and is recommended for intracellular protein expression and pathway engineering. B. subtilisWEA originated from the eightfold extracellular protease-deficient strain B. subtilisWB800N and is particularly constructed for secretory protein production.Both strains are suitable hosts for boththefood grade low copy expression vector pTTB1and the high copy expression vector pTTB2.TEA: trpC2ydcDE::Pxyl-ycdEWEA:nprE aprE epr bpr mpr::ble nprB::bsrΔvpr wprA::hyg cm::neoydcDE::Pxyl-ycdE; NeoR
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