pTTB1枯草芽孢杆菌食品级表达载体质粒Bacillus_subtilis_Food_Grade expression vector BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥59865
- 货 号：pTTB1枯草芽孢杆菌食品级表达载体质粒Bacillus_subtilis_Food_Grade expression vector
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pTTB1枯草芽孢杆菌食品级表达载体质粒Bacillus_subtilis_Food_Grade expression vector
The BacillusFood Grade Selection Systemprovidesthe following features:-Stable high-or low-level expression without addition of any antibiotics-All DNA contained in the final expression system is derived from B. subtilis-No endotoxins are produced-No inclusion bodies are formed-Protease-deficient strain for producing secretory enzymes is availableThe FoodGradeSelection SystemThe BacillusFoodGrade Selection Systemis based on the interplay of anendogenous BacillustoxinEndoAand its antitoxinEndoB.EndoA (encoded by ydcE) is an endoribonuclease that specifically cleaves mRNA at a five Base UACAU sequence(Pellegrini et al.,2005, Park et al.,2011). During normal growth conditions EndoA is inactivated by forming a heterohexameric complex with its cognate antitoxin EndoB (encoded by ydcD; Simanshu et al., 2013).Since the antitoxin is relatively unstable, it is essential for the cell to continuously produce sufficient amounts of EndoB to inactivate the morestable toxin.These characteristics are utilized for the here describedselection system.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
The BacillusFood Grade Selection Systemprovidesthe following features:-Stable high-or low-level expression without addition of any antibiotics-All DNA contained in the final expression system is derived from B. subtilis-No endotoxins are produced-No inclusion bodies are formed-Protease-deficient strain for producing secretory enzymes is availableThe FoodGradeSelection SystemThe BacillusFoodGrade Selection Systemis based on the interplay of anendogenous BacillustoxinEndoAand its antitoxinEndoB.EndoA (encoded by ydcE) is an endoribonuclease that specifically cleaves mRNA at a five Base UACAU sequence(Pellegrini et al.,2005, Park et al.,2011). During normal growth conditions EndoA is inactivated by forming a heterohexameric complex with its cognate antitoxin EndoB (encoded by ydcD; Simanshu et al., 2013).Since the antitoxin is relatively unstable, it is essential for the cell to continuously produce sufficient amounts of EndoB to inactivate the morestable toxin.These characteristics are utilized for the here describedselection system.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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