639-V cell line细胞株ACC-413 BioVector NTCC质粒载体菌种细胞基因保藏中心

639-V cell line细胞株ACC-413
ACC 413

Cell line:
639-V
DSMZ no.:
ACC 413
Species:
human (Homo sapiens)
Cell type:
ureter transitional cell carcinoma
Origin:
established from the transitional cell carcinoma (small, grade III) removed from the left ureter of a 69-year-old white man in 1973
Reference(s):
14506, 14505
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
epithelial-like cells growing adherently in monolayers;
Image:

Medium:
90% Dulbecco's MEM + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/25 cm2; split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 30 hours
Harvest:
cell harvest of ca. 6 x 106 cells/25 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de.
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed originally as human with IEF of AST, MDH later with STR typing
Cytogenetics:
human hyperdiploid karyotype with 24% polyploidy - 55(50-59)<2n>XX, +1, +3, +5, +6, +8, +11, +12, +14, -15, +20, -21, add(1)(p?21), i(5p), del(6)(q13-14), i(7p), add(8)(p2?), add(11)(p11), del(12)(q21q24), add(18)(q23) - additional copies of 6q-, i(7p), 8p+, der(18) markers present in sidelines together with del(7)(p13-14)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV

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