HEK293-S cell line细胞株HEK293S BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥39865
- 货 号：HEK293-S cell line细胞株HEK293S
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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HEK293-S cell line细胞株HEK293S
| Parental Line | Human embryonic kidney cells |
| Host | Human |
| Tissue | Kidney |
| Model | Immortalised Line |
| Relevance | The human embryonic kidney (HEK) 293 cell line and its derivatives are used in experiments ranging from signal transduction and protein interaction studies over viral packaging to rapid small-scale protein expression and biopharmaceutical production. The original 293 cells were derived in 1973 from the kidney of an aborted human embryo of unknown parenthood by transformation with sheared Adenovirus 5 DNA. The human embryonic kidney cells at first seemed recalcitrant to transformation. After many attempts, cell growth took off only several months after the isolation of a single transformed clone. A 4-kbp adenoviral genome fragment is known to have integrated in chromosome 19 and encodes for the E1A/E1B proteins, which interfere with the cell cycle control pathways and counteract apoptosis. Cytogenetic analysis established that the 293 line is pseudotriploid. HEK 293S cell line is adapted for culture in suspension. |
| Production Details | Hek 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA |
| Conditional | No |
| Research Area | Cancer |
| Recommended Growing Conditions | EMEM (EBSS) + 2mM Glutamine + 1% Non-Essential Amino Acids (NEAA) + 10% FCS |
| Notes | These HEK 293S have been whole-genome re-sequenced at early passage. The article by Lin et al al, allows user friendly visualisation of the genome data. The genome structure composition is in steady state when standard cell culturing conditions are used. |
Supplier来源:BioVector NTCC Inc.
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