HPB-ALL人T细胞白血病细胞株-BioVector NTCC保藏中心

Cell line:

HPB-ALL

NTCC no.:

NTCC-ACC 483

Species validation:

human (Homo sapiens)

Cell type:

T cell leukemia

Origin:

established from the peripheral blood of a 14-year-old boy with ALL and thymoma at diagnosis in 1973. Exome and RNA sequence data are available

NTCC Cell Culture Data:

Morphology:

round to polymorph cells growing singly or in clusters in suspension;

Image

Medium:

80-90% RPMI 1640 + 10-20% h.i. FBS

Subculture:

seed out at ca. 1 x 106 cells/ml; after thawing, we recommend to use initially 24-well-plates and 20% FBS; cell viability may be as low as 50% after thawing and cells may do not grow during the first week, then recover slowly; in this first week do not dilute, only exchange spent medium; when growing is resumed, split saturated culture 1:2 to 1:3 every 2-3 days

Incubation:

at 37 °C with 5% CO2

Doubling time:

ca. 50 hours

Harvest:

maximum density of about 4 x 106 cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:

Mycoplasma:

negative in microbiological culture, RNA hybridization, PCR assays

Immunology:

CD2 +, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

pseudodiploid human karyotype with 8% polyploidy - 46<2n>XY, der(1)t(1;16)(q22;p11-12)add(16)(p13), del(2)(p24), del(3)(p11), der(5)t(1;5)(q22-24;q32-33), r(16)(?p12?q12) - sideline with -3 instead of del(3) - resembles published karyotype

Molec. Genetics:

expression of TLX3 confirmed by RT-PCR

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

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