Toronto KnockOut (TKO) CRISPR Library - Version 3人源基因编辑gRNA文库-慢病毒骨架载体 (Pooled Libraries #90294, #125517) BioVector NTCC Inc.

Toronto KnockOut (TKO) CRISPR Library - Version 3人源基因编辑gRNA文库-慢病毒骨架载体
(Pooled Libraries #90294, #125517) BioVector NTCC质粒载体菌种细胞基因保藏中心

PURPOSE
Human CRISPR lentiviral library that has 70,948 guides (4 guides/gene) targeting 18,053 protein-coding genes.

VECTOR BACKBONE
Available in either a 1 vector (lentiCRISPRv2 backbone) or 2 vector (pLCKO2 backbone) system.
1 vector system (lentiCRISPRv2)
This backbone encodes Cas9.
2 vector system (pLCKO2)
A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pAAVS1-tet-iCas9-BFP2 ( #125519), lenti-Cas9-2A-Blast ( #73310), or with cell lines already expressing Cas9.LIBRARY DETAILS
SpeciesHuman
Genes targeted18,053
gRNAs70,948
Controls142 guides targeting LacZ, EGFP, and luciferase
Lentiviral Generation3rd
LIBRARY SHIPPING
This library will be delivered as a pooled DNA library in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

Volume∼20µL
Concentration50ng/µL
RESOURCE INFORMATION
Protocols
CRISPR Screen Protocol (244 KB)
Depositor Data
TKOv3 Guide Sequences (3.4 MB)
Please also visit the  Moffat Toronto KnockOut (TKO) library website for the most up-to-date information.

The Moffat Lab also provides a  gRNA viewer, that displays gRNA sequence information for a specific gene.
DEPOSITOR COMMENTS
The Toronto KnockOut Library v3 (TKOv3) contains 70,948 gRNAs targeting 18,053 protein coding genes (4 gRNAs/gene) with 142 control gRNAs targeting EGFP, LacZ, and luciferase (71,090 total).

The gRNAs in this library are optimized for editing efficiency using empirical data described in the associated article. As a result, the number of gRNAs per gene is decreased to 4 based on the finding that there are diminishing benefits to more gRNAs per gene beyond four. The optimized library size of TKOv3 offers the benefit of improved accuracy, efficiency and scalability for CRISPR screens.

This library is available in two formats: 1) as a one-component library that contains Cas9, alleviating the need to include a separate Cas9 and 2) as a two-component without Cas9, that can be utilized in cells that stably express Cas9.

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net