敲除wecA的大肠杆菌CLM37: DwecA E.coli菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：敲除wecA的大肠杆菌CLM37: DwecA E.coli菌株
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敲除wecA的大肠杆菌CLM37: DwecA E.coli菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Glycoengineered E. coli strains CLM37: DwecA
GlycoengineeringschematicinE.coli.TheE.colistrainsCLM37orSDB1arecommonlyusedforheterologousglycanassemblyandtransfer.AplasmidencodingforthebiosyntheticmachineryofaparticularglycanistransferredintoE.coli.Theglycanisthenassembledsequentiallyontoamonophosphorylatedundecaprenolprecursor(1),flippedtotheperiplasm(2),andpolymerizedintoalipid-linkedpolysaccharide(3).Thelipid-linkedpolysaccharidecanbesubsequentlytransferredtotheoutercoresaccharidetogenerateanLPSmoleculebytheWaaLligase(4a)ortransferredbyanoligosacchryltransferasetoatargetprotein
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