SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified新型冠状病毒核衣壳蛋白抗体兔多抗

产品名称
SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified
经验证的应用
WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
应用说明
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
特异性
SARS CoV Nucleocapsid
Has cross-reactivity in ELISA and WB with
SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP Protein (BioVector 40588-V08B).
免疫原
Recombinant SARS-CoV Nucleoprotein / NP Protein (BioVector40143-V08B)
制备方法
Produced in rabbits immunized with purified, recombinant SARS-CoV Nucleoprotein / NP (BioVector40143-V08B; NP_828858.1; Met1-Ala422). SARS-CoV Nucleoprotein / NP specific IgG was purified by SARS-CoV Nucleoprotein / NP affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗体) 经验证的应用
应用 推荐稀释比/用量
WB 1:1000-1:5000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.
SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗体) 图片
Anti-SARS-NP rabbit polyclonal antibody at 1:2000 dilution.
Lane A: SARS-CoV NP Protein (BioVector40143-V08B) (30ng)
Lane B: SARS-CoV NP Protein (BioVector40143-V08B) (5ng)
Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (BioVector40143-V08B) (30ng)
Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (BioVector40143-V08B) (5ng)
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
SARS-CoV Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified: 别称
Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody
CoV Nucleocapsid 背景信息
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
研究领域
• Microbiology>>Pathogenic microorganism>>viruses>>Animal virus>>RNA virus>>+ssRNA virus>>Coronaviridae>>Coronavirus (CoV)>>CoV viral antigen
参考文献
1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
3. Li W. et al., 2005, Science. 309 (5742): 1864-8.



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