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基因敲降(敲低)逆病毒质粒pRetro-SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP shRNA

  • 价  格:¥0
  • 货  号:NTCC-V20191189
  • 产  地:北京
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SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP ORF mammalian expression plasmid (Codon Optimized): 产品信息
基因
种属 2019-nCoV
基因长度 1260 bp
序列特征 A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with QHD43423.2 except for the point mutations: G355A.
产品特征 Full length Clone DNA of SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP.
SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP ORF mammalian expression plasmid (Codon Optimized): 别称
coronavirus NP cDNA ORF Clone, 2019-nCoV; coronavirus Nucleocapsid cDNA ORF Clone, 2019-nCoV; coronavirus Nucleoprotein cDNA ORF Clone, 2019-nCoV; cov np cDNA ORF Clone, 2019-nCoV; ncov NP cDNA ORF Clone, 2019-nCoV; NCP-CoV Nucleocapsid cDNA ORF Clone, 2019-nCoV; novel coronavirus NP cDNA ORF Clone, 2019-nCoV; novel coronavirus Nucleocapsid cDNA ORF Clone, 2019-nCoV; novel coronavirus Nucleoprotein cDNA ORF Clone, 2019-nCoV; np cDNA ORF Clone, 2019-nCoV; nucleocapsid cDNA ORF Clone, 2019-nCoV; Nucleoprotein cDNA ORF Clone, 2019-nCoV
CoV Nucleocapsid 背景信息
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
研究领域
Microbiology>>Pathogenic microorganism>>viruses>>Animal virus>>RNA virus>>+ssRNA virus>>Coronaviridae>>Coronavirus (CoV)>>CoV viral antigen
参考文献
1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
Li W. et al., 2005, Science. 309 (5742): 1864-8.

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