EBY100酵母表面展示菌株-抗性基因型-Biovector NTCC典型培养物保藏中心

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EBY100 yeast strain酵母表面展示宿主菌 / 感受态细胞

Cat# NTCC-EBY100

Yeast NTCC^TMEBY100 strain

NTCC^TMEBY100 expresses the AGA1 gene under control of the GAL1 promoter. It was created by integrating the vector pIU211 into the AGA1 locus of the Saccharomyces cerevisiae strain BJ5465 (MATa ura 3-52 trp 1 leu2Δ1 his3Δ200 pep4:HIS3 prb1Δ1.6R can1 GAL)(Boder and Wittrup, 1997). pIU211 contains the AGA1 gene regulated by the GAL promoter and a URA3 selectable marker. The vector was linearized for integration at AGA1 using BsiW I. BsiW I cuts in the AGA1 gene, ensuring that the construct integrates at the AGA1 locus.

To transform NTCC^TMEBY100 you will need the following reagents.

• Minimal dextrose plates containing leucine and tryptophan

• YPD medium

• Minimal dextrose plates containing leucine

• 20% Glucose

• 0.5-5 μg plasmid DNA (DNA may be isolated from E. coli using your method of

choice)

Transformation of NTCC^TMEBY100

Follow the general steps below to transform NTCC^TMEBY100.

1. Take the glycerol stock of NTCC^TMEBY100 and streak out on a minimal dextrose plate

containing leucine and tryptophan. Incubate at 30°C until colonies appear (1-2 days).

2. Use your method of choice to prepare competent cells and transform with pYD1 or

pYD1 containing your gene of interest.

3. Plate the transformation reaction (50-150 μl) on minimal dextrose plates containing

leucine. Incubate the plates at 30°C for 2 to 4 days until single colonies appear.

Once you have transformants, you are ready to test for display of your fusion protein.

Proceed to the next page to induce expression of your fusion protein.

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标签：以下资源由Biovector Inc. 质粒载体菌株细胞株基因保藏中心独家供应并发布，侵权必究。Biovector,Inc质粒载体菌株细胞株基因保藏中心列表网址http://biovector.blog.163.com质粒载体索取报价价格优惠售价出售图谱序列酶切位点转化构建求购购买订购 sequence map plasmid vector gene strain order ship 抗性 resistance restriction site

产品分类

原核表达载体

pYIP5
pDR195
pPICZα A
pHIC-PI
pRS426gal
pYEPlac112
pYX212
pFLD/CAT
pPICZα B
pESC-Leu
pYES2/CT
pYES2-EGFP
pMET C
pPink-LC
pGAPZα C
pGAPZ B
pPIC9K
pPIC9
SUMO protease
pGADT7
pYES2
pPIC ZαD
Ycplac33
p416GFD
pYRP7
pRS41H
pRS316
pESC-His
pYES2-NTC
pMETαC
pMET B
pPink-HC
pGAPZα B
pGAPZ A
pAO815
pPIC9k-His
pPICZ A
BFD15
pPIC ZαGB
pYEPlac195
pADH2
pYCP211
pFA6a-FGP(S65T)-kanMX6
pESC-URA
pYES6/CT
pYES2-NTA
pMETαB
pMET A
pHIL-D2
pGAPZα A
pPICZα C
pPIC3.5K
pAUR123
pPICZ C
LA503
pPIC ZαFC
Ycp22lac-EGFP
pYIP211
pYES2-kan
pRS426
pESC-TRP
pYES3/CT
pYES2-NTB
pMETαA
pPinkα-HC
pHIL-S1
pGAPZ C
pPICZ B
pPIC3.5
pSH47
pSH63
pSH65
pGBKT7
pHis2
pHisSi-1
pDest22
pGBKT7-53
pMyr
pGADT7-T
p53blue
pSos MAFB
pSos
p53his
pCL1
pGBKT7-Lam
pACT2 AD
pGAG424
pDest32
pBridge
pYD1
pDisplay
AH109
PichiaPink Strain3
SMD1168H
X-33
INVSC1
PichiaPink Strain2
SMD1163
KM71
SMD1168
PichiaPink Strain1
KM71H
PichiaPink Strain4
EBY100
GS115