NR-49806 Escherichia coli K-12, Strain IM08B金葡菌限制修饰系统改造型大肠杆菌菌株BioVector NTCC典型培养物保藏中心

NR-49806 Escherichia coli K-12, Strain IM08B strain金葡菌限制修饰系统改造型菌株

BioVector NTCC典型培养物保藏中心

NR-49806??Escherichia coli, K-12, Strain IM08B(Bacteria)|Escherichia coli|K-12, Strain IM08B|-60°C or colder|I Monk, T Stinear, T Foster

Escherichia coli (E. coli) K-12, strain IM08B is an insertion mutant produced in E. coli K-12, strain DC10B via recombination-mediated genetic engineering (recombineering), and contains the hsdM (methylase) and hsdS (specificity) genes from Staphylococcus aureus (S. aureus), strain NRS384 clonal complex 8 (CC8).

E. coli K-12, strain DC10B is a universal host for constructing plasmids for introduction into staphylococci and was deposited as Δdcm. The lack of methylation allows plasmid DNA to bypass a conserved type IV restriction-modification (RM) barrier in staphylococci which has been identified as a major barrier to transformation with foreign DNA. E. coli K-12, strain IM08B was deposited as resistant to streptomycin.

Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth containing 25 ?g per mL streptomycin supplemented with 10% glycerol.

大肠杆菌（大肠杆菌） K-12，菌株IM08b是在 e中产生的插入突变体. COLI K-12，菌株DC10B通过重组介导的基因工程（重组化），并含有 HSDM （甲基酶）和 HSDS （特异性）基因来自金黄色葡萄球菌（金黄色葡萄球菌），菌株NRS384克隆复合物8（CC8）. e. COLI K-12，菌株DC10B是用于构建用于引入葡萄球菌的质粒的通用宿主，并沉积为δ2DCM/ i>.缺乏甲基化允许质粒DNA在葡萄球菌中绕过保守的IV型限制性改性（RM）屏障，该葡萄球菌被鉴定为用外来DNA转化的主要屏障. e. Coli K-12，菌株IM08b被沉积为耐链霉素. 每个小瓶在含有10％甘油补充有10％甘油的胰蛋白酶肉汤中含有大约0.5ml的细菌培养物中的细菌培养物.

Description: Several S. aureus restriction-system-modified E. coli strains, such as IM08B and ALC7885, probably can also be used for the restriction modification of the pnCasSA–BEC plasmid.

BioSafety Level:3

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