| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pMD2.G, pMD2G |
| 质粒类型: | 慢病毒包装载体质粒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5824bp |
| 5' 测序引物及序列: | CMV Fwd: CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 筛选标记: | -- |
| 备注: | Envelope plasmid. Known to work with most Aebischer and Trono lab lentiviral vectors, as well as the pLKO system。慢性毒包装质粒 |
| 产品目录号: | -- |
| 稳定性: | 稳定 |
| 组成型: | 非组成型 |
| 病毒/非病毒: | 慢病毒 |
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pMD2G载体基本信息
pMD2G载体质粒图谱和多克隆位点信息
pMD2G载体简介
pMD2G载体序列
LOCUS pMD2.G 5824 bp DNA SYN
DEFINITION pMD2.G
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biovector.net/
COMMENT VNTAUTHORNAME|biovector.net|
FEATURES Location/Qualifiers
source 1..5824
/organism="pMD2.G"
/mol_type="other DNA"
misc_feature complement(40..59)
/label="SV40pro_F_primer"
promoter 162..738
/label="CMV_immearly_promoter"
misc_feature 241..528
/label="CAG_enhancer"
misc_feature 695..715
/label="CMV_fwd_primer"
promoter 696..765
/label="CMV_promoter"
promoter 708..827
/label="CMV2_promoter"
misc_feature 739..758
/label="pCEP_fwd_primer"
misc_feature 741..765
/label="LNCX_primer"
misc_feature 1015..1398
/label="bGlob_int"
gene 1450..2973
/label="vsv-G"
/gene="vsv-G"
CDS 1450..2985
/label="ORF frame 1"
misc_feature 2950..2982
/label="VSVG Tag"
rep_origin complement(4055..4674)
/label="pBR322_origin"
gene complement(4829..5689)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4829..5689)
/label="ORF frame 1"
promoter complement(5731..5759)
/label="AmpR_promoter"
ORIGIN
1 ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa
61 aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg
121 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
841 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
901 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
961 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
1021 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
1081 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
1141 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
1201 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
1261 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
1321 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
1381 ctggcccatc actttggcaa agcacgtgag atctgaattc aacagagatc gatctgtttc
1441 cttgacacta tgaagtgcct tttgtactta gcctttttat tcattggggt gaattgcaag
1501 ttcaccatag tttttccaca caaccaaaaa ggaaactgga aaaatgttcc ttctaattac
1561 cattattgcc cgtcaagctc agatttaaat tggcataatg acttaatagg cacagccata
1621 caagtcaaaa tgcccaagag tcacaaggct attcaagcag acggttggat gtgtcatgct
1681 tccaaatggg tcactacttg tgatttccgc tggtatggac cgaagtatat aacacagtcc
1741 atccgatcct tcactccatc tgtagaacaa tgcaaggaaa gcattgaaca aacgaaacaa
1801 ggaacttggc tgaatccagg cttccctcct caaagttgtg gatatgcaac tgtgacggat
1861 gccgaagcag tgattgtcca ggtgactcct caccatgtgc tggttgatga atacacagga
1921 gaatgggttg attcacagtt catcaacgga aaatgcagca attacatatg ccccactgtc
1981 cataactcta caacctggca ttctgactat aaggtcaaag ggctatgtga ttctaacctc
2041 atttccatgg acatcacctt cttctcagag gacggagagc tatcatccct gggaaaggag
2101 ggcacagggt tcagaagtaa ctactttgct tatgaaactg gaggcaaggc ctgcaaaatg
2161 caatactgca agcattgggg agtcagactc ccatcaggtg tctggttcga gatggctgat
2221 aaggatctct ttgctgcagc cagattccct gaatgcccag aagggtcaag tatctctgct
2281 ccatctcaga cctcagtgga tgtaagtcta attcaggacg ttgagaggat cttggattat
2341 tccctctgcc aagaaacctg gagcaaaatc agagcgggtc ttccaatctc tccagtggat
2401 ctcagctatc ttgctcctaa aaacccagga accggtcctg ctttcaccat aatcaatggt
2461 accctaaaat actttgagac cagatacatc agagtcgata ttgctgctcc aatcctctca
2521 agaatggtcg gaatgatcag tggaactacc acagaaaggg aactgtggga tgactgggca
2581 ccatatgaag acgtggaaat tggacccaat ggagttctga ggaccagttc aggatataag
2641 tttcctttat acatgattgg acatggtatg ttggactccg atcttcatct tagctcaaag
2701 gctcaggtgt tcgaacatcc tcacattcaa gacgctgctt cgcaacttcc tgatgatgag
2761 agtttatttt ttggtgatac tgggctatcc aaaaatccaa tcgagcttgt agaaggttgg
2821 ttcagtagtt ggaaaagctc tattgcctct tttttcttta tcatagggtt aatcattgga
2881 ctattcttgg ttctccgagt tggtatccat ctttgcatta aattaaagca caccaagaaa
2941 agacagattt atacagacat agagatgaac cgacttggaa agtaactcaa atcctgcaca
3001 acagattctt catgtttgga ccaaatcaac ttgtgatacc atgctcaaag aggcctcaat
3061 tatatttgag tttttaattt ttatggaatt caccccacca gtgcaggctg cctatcagaa
3121 agtggtggct ggtgtggcta atgccctggc ccacaagttt cactaagctc gcttccttgc
3181 tgtccaattt ctattaaagg ttccttggtt ccctaagtcc aactactaaa ctgggggata
3241 ttatgaaggg ccttgagcat ctggattctg cctaataaaa aacatttatt ttcattgcaa
3301 tgatgtattt aaattatttc tgaatatttt actaaaaagg gaatgtggga ggtcagtgca
3361 tttaaaacat aaagaaatga agagctagtt caaaccttgg gaaaatacac tatatcttaa
3421 actccatgaa agaaggtgag gctgcaaaca gctaatgcac attggcaaca gccctgatgc
3481 ctatgcctta ttcatccctc agaaaaggat tcaagtagag gcttgatttg gaggttaaag
3541 tttggctatg ctgtatttta cattacttat tgttttagct gtcctcatga atgtcttttc
3601 actacccatt tgcttatcct gcatctctca gccttgactc cactcagttc tcttgcttag
3661 agataccacc tttcccctga agtgttcctt ccatgtttta cggcgagatg gtttctcctc
3721 gcctggccac tcagccttag ttgtctctgt tgtcttatag aggtctactt gaagaaggaa
3781 aaacaggggg catggtttga ctgtcctgtg agcccttctt ccctgcctcc cccactcaca
3841 gtgacccgga atccctcgac atggcagtct agcactagtg cggccgcaga tctgcttcct
3901 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
3961 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
4021 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
4081 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
4141 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
4201 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
4261 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
4321 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
4381 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
4441 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
4501 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
4561 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
4621 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
4681 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
4741 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
4801 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
4861 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
4921 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
4981 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
5041 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
5101 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
5161 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
5221 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
5281 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
5341 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
5401 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
5461 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
5521 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
5581 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
5641 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
5701 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
5761 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
5821 acgt
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