pESC-Leu质粒载体图谱序列抗性说明书价格Biovector公司

LOCUS pESC-LEU 7758 bp DNA circular SYN 10-OCT-2005
DEFINITION Cloning vector pESC-LEU, complete sequence.
ACCESSION AF063849
VERSION AF063849.1 GI:3228559
SOURCE Cloning vector pESC-LEU.
ORGANISM Cloning vector pESC-LEU
artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..7758
/organism="Cloning vector pESC-LEU"
/mol_type="genomic DNA"
/specific_host="Escherichia coli K12"
/db_xref="taxon:76666"
/vntifkey="98"
CDS complement(5359..6216)
/vntifkey="4"
/label=AmpR
rep_origin 6350..7505
/vntifkey="33"
/label=2\micron\ori
CDS complement(663..1766)
/vntifkey="4"
/label=LEU2
/note="yeast LEU2 ORF"
terminator complement(3012..3176)
/vntifkey="43"
/label=ADH1\terminator
terminator 4165..4354
/vntifkey="43"
/label=CYC1\terminator
rep_origin complement(2606..2912)
/vntifkey="33"
/label=f1\ori
promoter 3607..4061
/vntifkey="29"
/label=GAL1\promoter
promoter complement(3395..3606)
/vntifkey="29"
/label=GAL10\promoter
misc_feature 4103..4138
/vntifkey="21"
/label=c-myc\tag
misc_feature complement(3325..3351)
/vntifkey="21"
/label=FLAG\tag
misc_feature complement(3307..3369)
/vntifkey="21"
/label=MCS1
misc_feature 4063..4160
/vntifkey="21"
/label=MCS2
rep_origin 4541..5208
/vntifkey="33"
/label=pUC\ori
BASE COUNT 2201 a 1663 c 1649 g 2245 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc
241 accattatgg gaaatgcttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca
301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat
361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc
421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc
481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt
541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg
601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct
661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg
721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct
781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac
841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat
901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc
961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg
1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca
1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc
1141 acagtttttc tccataatct tgaagaggcc aaaagattag ctttatccaa ggaccaaata
1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact
1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc
1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca
1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt
1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca
1501 ctaccggtac cccatttagg accagccaca gcacctaaca aaacggcatc aaccttcttg
1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca
1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga
1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc
1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata
1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat
1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat
1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct
1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca
2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat
2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga
2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg
2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt
2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg
2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta
2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa
2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg
2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
3001 cagctgaatt ggagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga
3061 gttactcaag aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac
3121 acttattttt tttataactt atttaataat aaaaatcata aatcataaga aattcgctta
3181 tttagaagtg tcaacaacgt atctaccaac gatttgaccc ttttccatct tttcgtaaat
3241 ttctggcaag gtagacaagc cgacaacctt gattggagac ttgaccaaac ctctggcgaa
3301 gaattgttaa ttaagagctc agatcttatc gtcgtcatcc ttgtaatcca tcgatactag
3361 tgcggccgcc ctttagtgag ggttgaattc gaattttcaa aaattcttac tttttttttg
3421 gatggacgca aagaagttta ataatcatat tacatggcat taccaccata tacatatcca
3481 tatacatatc catatctaat cttacttata tgttgtggaa atgtaaagag ccccattatc
3541 ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta
3601 tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc
3661 tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca
3721 ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt
3781 ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg
3841 ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt
3901 ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc
3961 aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg
4021 ttaatatacc tctatacttt aacgtcaagg agaaaaaacc ccggatccgt aatacgactc
4081 actatagggc ccgggcgtcg acatggaaca gaagttgatt tccgaagaag acctcgagta
4141 agcttggtac cgcggctagc taagatccgc tctaaccgaa aaggaaggag ttagacaacc
4201 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
4261 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
4321 aaccttgctt gagaaggttt tgggacgctc gaagatccag ctgcattaat gaatcggcca
4381 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
4441 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
4501 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
4561 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
4621 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
4681 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
4741 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
4801 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
4861 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
4921 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
4981 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
5041 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
5101 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
5161 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
5221 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
5281 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
5341 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
5401 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
5461 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
5521 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
5581 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
5641 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
5701 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
5761 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
5821 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
5881 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
5941 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
6001 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
6061 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
6121 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
6181 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
6241 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
6301 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg
6361 tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
6421 tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa
6481 tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
6541 gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca
6601 aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa
6661 caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata
6721 actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc
6781 ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg
6841 tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat
6901 actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg
6961 gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt
7021 ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag taatactaga
7081 gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga
7141 tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa
7201 tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg
7261 gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta
7321 tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg
7381 cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat
7441 ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt
7501 aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg
7561 atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt
7621 ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc atttcctttg
7681 atattggatc atactaagaa accattatta tcatgacatt aacctataaa aataggcgta
7741 tcacgaggcc ctttcgtc
//