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AQ10180 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心
Strain菌株名:AQ10180
Plasmid Integrated整合质粒:
Plasmid autonomous:
Prophage:λ+ λp[sfiA::lac cI(Ind-)]
编号:ME9586
Genotype基因型:recG162 λp[sfiA::lac cI(Ind-)] rpsL Δ(pro-lac) pro zic::Tn10
Parent donor:AQ10070
Parent recipient:AQ8107
Sex:F-
Culture Condition培养条件:Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml).
Methods:TD./PI
Latest marker:UVs, recG162
Selection筛选条件:
Other remarks:Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Supplier:
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
Strain菌株名:AQ10180
Plasmid Integrated整合质粒:
Plasmid autonomous:
Prophage:λ+ λp[sfiA::lac cI(Ind-)]
编号:ME9586
Genotype基因型:recG162 λp[sfiA::lac cI(Ind-)] rpsL Δ(pro-lac) pro zic::Tn10
Parent donor:AQ10070
Parent recipient:AQ8107
Sex:F-
Culture Condition培养条件:Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml), tetracycline (20μg/ml).
Methods:TD./PI
Latest marker:UVs, recG162
Selection筛选条件:
Other remarks:Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kogoma T., 1997, Micrbiol. Mol. Biol. Rev., 61, 212-238. Walker G. C., 1996, The SOS response of Escherichia coli, p. 1400-1416. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Supplier:
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
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