AQ10075 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心

AQ10075 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心
Strain菌株名：AQ10075
Plasmid Integrated整合质粒：
Plasmid autonomous：
Prophage：
编号:ME9580
Genotype基因型:pro zig::Tn10 polA12 recG258::miniTn10kan deoB (or deoC) thyA metB1 trpA9605 his-29 ilv
Parent donor：AQ1524
Parent recipient:AQ8130
Sex：F-
Culture Condition培养条件:Recommended medium:
M9 salts glucose medium supplemented with Trp (100μg/ml), His (100μg/ml), Ile (100μg/ml), Val (100μg/ml) Met (100μg/ml), Pro (80μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Methods:TD./PI
Latest marker:Ts, UVs, polA12
Selection筛选条件:
Other remarks:Possible Ls (Srm) strain: sensitive for rich media. Kogoma and Meyenburg., 1983, EMBO J., 2, 463-468. Ogawa et al., 1984, Proc. Natl. Acad. Sci. USA., 81, 1040-1044.
Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.


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