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AQ9717 E.coli mutant大肠杆菌基因突变株-BioVector保藏中心
Strain菌株名:AQ9717
Plasmid Integrated整合质粒:
Plasmid autonomous:
Prophage:
编号:ME9556
Genotype基因型:thi-1 rpsL31 galK2 supE44 kdg-51 mtl-1 xyl-5 ara-14 lacY1 zga::Tn10 deoB (orC) tsx-33 thyA recN1502::Tn5 his-4 argE3 leuB6 thr-1 proA2
Parent donor:AQ5786
Parent recipient:AQ8224
Sex:F-
Culture Condition培养条件:Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Methods:TD./PI
Latest marker:low thymide, thyA
Selection筛选条件:
Other remarks:Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Supplier:
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
Strain菌株名:AQ9717
Plasmid Integrated整合质粒:
Plasmid autonomous:
Prophage:
编号:ME9556
Genotype基因型:thi-1 rpsL31 galK2 supE44 kdg-51 mtl-1 xyl-5 ara-14 lacY1 zga::Tn10 deoB (orC) tsx-33 thyA recN1502::Tn5 his-4 argE3 leuB6 thr-1 proA2
Parent donor:AQ5786
Parent recipient:AQ8224
Sex:F-
Culture Condition培养条件:Recommended medium:
M9 salts glucose medium supplemented with Arg (100μg/ml), Thr (100μg/ml), Leu (100μg/ml), Trp (100μg/ml), His (100μg/ml), Pro (100μg/ml), Thymine (20μg/ml), Thiamine (2μg/ml).
Methods:TD./PI
Latest marker:low thymide, thyA
Selection筛选条件:
Other remarks:Further information: Kowalczykowski et al., 1994, Microbiol. Rev., 58, 401-465.
Lloyd R. G. and Brookslow K., Homologous recombination, p. 2236-2255. In Neidhaldt et al. (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., American Society for Microbiolgy, Waschington, D. C.
Supplier:
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
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